glutamate + NAD(P)+ => alpha-ketoglutarate + NH4+ + NAD(P)H + H+ [GLUD1]

Stable Identifier
R-HSA-70600
Type
Reaction
Species
Homo sapiens
Compartment
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Mitochondrial glutamate dehydrogenase 1 (GLUD1) catalyzes the reversible reaction of glutamate and NAD(P)+ to form 2-oxoglutarate, NAD(P)H + H+, and ammonia (Fang et al. 2002). Mature GLUD1 protein lacks the 53 aminoterminal residues of the nascent protein (Julliard and Smith 1979), which function as a mitochondrial import signal. The active form of the enzyme is a hexamer, allosterically activated by ADP and inhibited by GTP (Fang et al. 2002; Smith et al. 2002).

Literature References
PubMed ID Title Journal Year
429360 Partial amino acid sequence of the glutamate dehydrogenase of human liver and a revision of the sequence of the bovine enzyme

Julliard, JH, Smith, EL

J Biol Chem 1979
11903050 Expression, purification and characterization of human glutamate dehydrogenase (GDH) allosteric regulatory mutations

Smith, TJ, MacMullen, CM, Hsu, BY, Fang, J, Poncz, M, Stanley, CA

Biochem J 2002
12054821 The structure of apo human glutamate dehydrogenase details subunit communication and allostery

Smith, TJ, Wu, J, Schmidt, T, Fang, J, Stanley, CA, Siuzdak, G

J Mol Biol 2002
Participants
Participates
Catalyst Activity

glutamate dehydrogenase [NAD(P)+] activity of GLUD hexamer [mitochondrial matrix]

This event is regulated
Negatively by
Positively by
Orthologous Events
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