Reactome | PXLP-K314-GPT transaminates PYR to form L-Ala

PXLP-K314-GPT transaminates PYR to form L-Ala

Stable Identifier

R-HSA-70524

Type

Reaction [transition]

Species

Homo sapiens

Compartment

cytosol

Synonyms

pyruvate + glutamate <=> alanine + alpha-ketoglutarate [GPT]

ReviewStatus

5/5

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PXLP-K314-GPT transaminates PYR to form L-Ala

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Cytosolic glutamic-pyruvate transaminase (alanine aminotransferase) (GPT) catalyzes the reversible reaction of pyruvate and glutamate to form alanine and 2-oxoglutarate (alpha-ketoglutarate) (Sohocki et al. 1997; Yang et al. 2002). The active form of the enzyme is a dimer (Ishiguro et al. 1991) and is inferred to have a molecule of pyridoxal phosphate associated with each monomer. This reaction allows the synthesis of alanine from intermediates of glucose metabolism in a well-fed person. Under fasting conditions, alanine, derived from protein breakdown, can be converted to pyruvate and used to synthesize glucose via the gluconeogenic pathway in liver, or fully oxidized via the TCA cycle in other tissues.

Literature References

PubMed ID	Title	Journal	Year
9119391	Human glutamate pyruvate transaminase (GPT): localization to 8q24.3, cDNA and genomic sequences, and polymorphic sites. Sohocki, MM, Sullivan, LS, Harrison, WR, Sodergren, EJ, Elder, FF, Weinstock, G, Tanase, S, Daiger, SP	Genomics	1997
1931970	Complete amino acid sequence of human liver cytosolic alanine aminotransferase (GPT) determined by a combination of conventional and mass spectral methods Ishiguro, M, Takio, K, Suzuki, M, Oyama, R, Matsuzawa, T, Titani, K	Biochemistry	1991
11863375	cDNA cloning, genomic structure, chromosomal mapping, and functional expression of a novel human alanine aminotransferase Yang, RZ, Blaileanu, G, Hansen, BC, Shuldiner, AR, Gong, DW	Genomics	2002