Reactome | PXLP-K314-GPT transaminates L-Ala to form PYR

PXLP-K314-GPT transaminates L-Ala to form PYR

Stable Identifier

R-HSA-70523

Type

Reaction [transition]

Species

Homo sapiens

Compartment

cytosol

Synonyms

alanine + alpha-ketoglutarate <=> pyruvate + glutamate [GPT]

ReviewStatus

5/5

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PXLP-K314-GPT transaminates L-Ala to form PYR

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Cytosolic glutamic-pyruvate transaminase (alanine aminotransferase) (GPT) catalyzes the reversible reaction of alanine and 2-oxoglutarate (alpha-ketoglutarate) to form pyruvate and glutamate (Sohocki et al. 1997; Yang et al. 2002). The active form of the enzyme is a dimer (Ishiguro et al. 1991) and is inferred to have a molecule of pyridoxal phosphate associated with each monomer. This reaction allows the synthesis of alanine from intermediates of glucose metabolism in a well-fed person. Under fasting conditions, alanine, derived from protein breakdown, can be converted to pyruvate and used to synthesize glucose via the gluconeogenic pathway in liver, or fully oxidized via the TCA cycle in other tissues.

Literature References

PubMed ID	Title	Journal	Year
9119391	Human glutamate pyruvate transaminase (GPT): localization to 8q24.3, cDNA and genomic sequences, and polymorphic sites. Harrison, WR, Daiger, SP, Sodergren, EJ, Tanase, S, Elder, FF, Weinstock, G, Sullivan, LS, Sohocki, MM	Genomics	1997
1931970	Complete amino acid sequence of human liver cytosolic alanine aminotransferase (GPT) determined by a combination of conventional and mass spectral methods Oyama, R, Suzuki, M, Takio, K, Ishiguro, M, Titani, K, Matsuzawa, T	Biochemistry	1991
11863375	cDNA cloning, genomic structure, chromosomal mapping, and functional expression of a novel human alanine aminotransferase Hansen, BC, Shuldiner, AR, Blaileanu, G, Yang, RZ, Gong, DW	Genomics	2002