GAPDH tetramers reduce 1,3BPG to GA3P

Stable Identifier
Reaction [transition]
Homo sapiens
(2R)-3-phospho-glyceroyl phosphate + H(+) + NADH => D-glyceraldehyde 3-phosphate + NAD(+) + phosphate
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The reversible reduction of 1,3BPG (1,3-bisphosphoglycerate) to form G3P (glyceraldehyde-3-phosphate) is catalyzed by cytosolic GAPDH (glyceraldehyde-3-phosphate dehydrogenase) tetramer.

There are multiple human GAPDH-like pseudogenes, but only one GAPDH gene expressed in somatic tissue (Benham and Povey 1989). Consistent with this conclusion, the homogeneous enzymes purified from various human tissues had indistinguishable physical and immunochemical properties (Heinz and Freimuller 1982), and studies of human erythrocytes of various ages suggested that variant forms of the enzyme arise as a result of post-translational modifications (Edwards et al. 1976). There is, however, an authentic second isoform of GAPDH whose expression is confined to spermatogenic cells of the testis (Welch et al. 2000).

Literature References
PubMed ID Title Journal Year
10714828 Human glyceraldehyde 3-phosphate dehydrogenase-2 gene is expressed specifically in spermatogenic cells.

Eddy, EM, Bunch, DO, Mori, C, O'Brien, DA, Magyar, PL, Welch, JE, Brown, PL

J Androl 2000
183598 Isozymes of glyceraldehyde-3-phosphate dehydrogenase in man and other mammals.

Edwards, YH, Harris, H, Clark, P

Ann Hum Genet 1976
3170585 Isolation and complete sequence of a functional human glyceraldehyde-3-phosphate dehydrogenase gene

Florence, B, Alexander, M, Denaro, M, Ercolani, L

J Biol Chem 1988
7144574 Glyceraldehyde-3-phosphate dehydrogenase from human tissues.

Freimüller, B, Heinz, F

Methods Enzymol 1983
Catalyst Activity

glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity of GAPDH tetramers [cytosol]

Orthologous Events
Cross References
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