D-glyceraldehyde 3-phosphate + orthophosphate + NAD+ <=> 1,3-bisphospho-D-glycerate + NADH + H+

Stable Identifier
Reaction [transition]
Homo sapiens
Glyceraldehyde 3 phosphate, NAD+, and orthophosphate react to form 1,3 bisphosphoglycerate and NADH + H+
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Cytosolic glyceraldehyde 3-phosphate dehydrogenase catalyzes the reversible reaction of glyceraldehyde 3-phosphate, orthophosphate, and NAD+ to form NADH + H+ and 1,3-bisphosphoglycerate, the first energy rich intermediate of glycolysis. The biochemical details of this reaction were worked out by C and G Cori and their colleagues (Taylor et al. 1948; Cori et al. 1948).

While there are multiple human glyceraldehyde 3-phosphate dehydrogenase-like pseudogenes, there is only one glyceraldehyde 3-phosphate dehydrogenase gene expressed in somatic tissue (Benham and Povey 1989; Heinz and Freimuller 1982; Ercolani et al. 1988), and studies of aged human erythrocytes suggest that variant forms of the enzyme arise as a result of post-translational modifications (Edwards et al. 1976). There is, however, an authentic second isoform of glyceraldehyde 3-phosphate dehydrogenase whose expression is confined to spermatogenic cells of the testis (Welch et al. 2000).

Literature References
PubMed ID Title Journal Year
2793178 Members of the human glyceraldehyde-3-phosphate dehydrogenase-related gene family map to dispersed chromosomal locations.

Benham, FJ, Povey, S

Genomics 1989
18910717 The prosthetic group of crystalline d-glyceraldehyde-3-phosphate dehydrogenase


J. Biol. Chem. 1948
18910716 Crystalline D-glyceraldehyde-3-phosphate dehydrogenase from rabbit muscle

Cori, CF, Slein, MW, Cori, GT

J Biol Chem 1948
10714828 Human glyceraldehyde 3-phosphate dehydrogenase-2 gene is expressed specifically in spermatogenic cells.

Eddy, EM, Bunch, DO, Mori, C, O'Brien, DA, Magyar, PL, Welch, JE, Brown, PL

J Androl 2000
183598 Isozymes of glyceraldehyde-3-phosphate dehydrogenase in man and other mammals.

Edwards, YH, Harris, H, Clark, P

Ann Hum Genet 1976
3170585 Isolation and complete sequence of a functional human glyceraldehyde-3-phosphate dehydrogenase gene

Florence, B, Alexander, M, Denaro, M, Ercolani, L

J Biol Chem 1988
7144574 Glyceraldehyde-3-phosphate dehydrogenase from human tissues.

Freimüller, B, Heinz, F

Methods Enzymol 1983
Event Information
Catalyst Activity

glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity of GAPDH tetramers [cytosol]

Orthologous Events
Cross References
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