Reactome | D-glyceraldehyde 3-phosphate + orthophosphate + NAD+ <=> 1,3-bisphospho-D-glycerate + NADH + H+

D-glyceraldehyde 3-phosphate + orthophosphate + NAD+ <=> 1,3-bisphospho-D-glycerate + NADH + H+

Stable Identifier

R-HSA-70449

Type

Reaction

Species

Homo sapiens

Compartment

cytosol

Synonyms

Glyceraldehyde 3 phosphate, NAD+, and orthophosphate react to form 1,3 bisphosphoglycerate and NADH + H+

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D-glyceraldehyde 3-phosphate + orthophosphate + NAD+ <=> 1,3-bisphospho-D-glycerate + NADH + H+

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Cytosolic glyceraldehyde 3-phosphate dehydrogenase catalyzes the reversible reaction of glyceraldehyde 3-phosphate, orthophosphate, and NAD+ to form NADH + H+ and 1,3-bisphosphoglycerate, the first energy rich intermediate of glycolysis. The biochemical details of this reaction were worked out by C and G Cori and their colleagues (Taylor et al. 1948; Cori et al. 1948).

While there are multiple human glyceraldehyde 3-phosphate dehydrogenase-like pseudogenes, there is only one glyceraldehyde 3-phosphate dehydrogenase gene expressed in somatic tissue (Benham and Povey 1989; Heinz and Freimuller 1982; Ercolani et al. 1988), and studies of aged human erythrocytes suggest that variant forms of the enzyme arise as a result of post-translational modifications (Edwards et al. 1976). There is, however, an authentic second isoform of glyceraldehyde 3-phosphate dehydrogenase whose expression is confined to spermatogenic cells of the testis (Welch et al. 2000).

Literature References

PubMed ID	Title	Journal	Year
2793178	Members of the human glyceraldehyde-3-phosphate dehydrogenase-related gene family map to dispersed chromosomal locations. Benham, FJ, Povey, S	Genomics	1989
18910717	The prosthetic group of crystalline d-glyceraldehyde-3-phosphate dehydrogenase VELICK, SF, TAYLOR, JF	J. Biol. Chem.	1948
18910716	Crystalline D-glyceraldehyde-3-phosphate dehydrogenase from rabbit muscle Cori, CF, Slein, MW, Cori, GT	J Biol Chem	1948
10714828	Human glyceraldehyde 3-phosphate dehydrogenase-2 gene is expressed specifically in spermatogenic cells. Eddy, EM, Bunch, DO, Mori, C, O'Brien, DA, Magyar, PL, Welch, JE, Brown, PL	J Androl	2000
183598	Isozymes of glyceraldehyde-3-phosphate dehydrogenase in man and other mammals. Edwards, YH, Harris, H, Clark, P	Ann Hum Genet	1976
3170585	Isolation and complete sequence of a functional human glyceraldehyde-3-phosphate dehydrogenase gene Florence, B, Alexander, M, Denaro, M, Ercolani, L	J Biol Chem	1988
7144574	Glyceraldehyde-3-phosphate dehydrogenase from human tissues. Freimüller, B, Heinz, F	Methods Enzymol	1983