CHEK2 phosphorylates TP53

Stable Identifier
Reaction [transition]
Homo sapiens
Phosphorylation of p53 by ATM-activated Chk2
Locations in the PathwayBrowser
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CHEK2 (Chk2) phosphorylates TP53 (p53) at serine residue S20 (Hirao et al. 2000, Shieh et al. 2000, Chehab et al. 2000). Phosphorylation of TP53 at serine residue S20 is necessary for DNA damage-induced TP53 stabilization as it compromises the interaction of TP53 with the ubiquitin ligase MDM2 (Chehab et al. 1999, Chehab et al. 2000). S20 phosphorylation is also required for the induction of TP53-dependent transcripts in response to DNA damage (Hirao et al. 2000).
Literature References
PubMed ID Title Journal Year
10673500 Chk2/hCds1 functions as a DNA damage checkpoint in G(1) by stabilizing p53.

Appel, M, Halazonetis, TD, Chehab, NH, Malikzay, A

Genes Dev 2000
10570149 Phosphorylation of Ser-20 mediates stabilization of human p53 in response to DNA damage

Halazonetis, TD, Malikzay, A, Stavridi, ES, Chehab, NH

Proc. Natl. Acad. Sci. U.S.A. 1999
10673501 The human homologs of checkpoint kinases Chk1 and Cds1 (Chk2) phosphorylate p53 at multiple DNA damage-inducible sites.

Tamai, K, Taya, Y, Ahn, J, Prives, C, Shieh, SY

Genes Dev 2000
10710310 DNA damage-induced activation of p53 by the checkpoint kinase Chk2.

Hirao, A, Elledge, SJ, Mak, TW, Yoshida, H, Liu, D, Matsuoka, S, Wakeham, A, Ruland, J, Kong, YY

Science 2000
Catalyst Activity

protein serine/threonine kinase activity of p-S,3T-CHEK2 [nucleoplasm]

Orthologous Events
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