ORC1 binds to the ORC(2-5) complex in the nucleus to form a stable ORC(1-5) complex (Radichev et al. 2006; Ghosh et al. 2011). ORC1 is necessary for the association of the ORC(2-5) complex to chromatin (Radichev et al. 2006). The ORC(2-5) complex exhibits a tightly autoinhibited conformation, with the winged-helix domain (WHD) of ORC2 completely blocking the central DNA-binding channel. Binding of ORC1 remodels the WHD of ORC2, moving it away from the central channel and partially relieving the autoinhibition (Cheng et al. 2020, Jaremko et al. 2020). Several dynamic structures of the ORC(1-5) complex have been revealed by cryo-electron microscopy (cryoEM), which depend on the presence of DNA and ATP (Jaremko et al. 2020).
Radichev, I, Zhao, Y, Vassilev, A, Kwon, SW, DePamphilis, ML
On, KF, Joshua-Tor, L, Thomas, DR, Stillman, B, Jaremko, MJ
Ghosh, S, Zhao, Y, Vassilev, AP, Zhang, J, DePamphilis, ML
Wang, X, Cheng, J, Gao, N, Zhai, Y, Hu, J, Li, N
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