ATP hydrolysis by NSF disassembles the cis-SNARE at the TGN

Stable Identifier
Reaction [transition]
Homo sapiens
Locations in the PathwayBrowser
SVG |   | PPTX  | SBGN
Click the image above or here to open this reaction in the Pathway Browser
The layout of this reaction may differ from that in the pathway view due to the constraints in pathway layout

NSF-dependent hydrolysis of ATP is required to disassociate the cis-SNARE complex, releasing the SNAREs for further rounds of membrane fusion (Mayer et al, 1996; Muller et al, 1999; Muller et al, 2002; Otto et al, 1997; Whiteheart et al, 2004; Yu et al, 1999; Zhao et al, 2012; Shah et al, 2015; reviewed in Sudhof and Rothman, 2009).

Literature References
PubMed ID Title Journal Year
9177194 Assembly and disassembly of a ternary complex of synaptobrevin, syntaxin, and SNAP-25 in the membrane of synaptic vesicles

Otto, H, Hanson, PI, Jahn, R

Proc. Natl. Acad. Sci. U.S.A. 1997
8620540 Sec18p (NSF)-driven release of Sec17p (alpha-SNAP) can precede docking and fusion of yeast vacuoles

Mayer, A, Wickner, W, Haas, A

Cell 1996
10445031 NSF N-terminal domain crystal structure: models of NSF function

Yu, RC, Jahn, R, Brunger, AT

Mol. Cell 1999
15037235 Multiple binding proteins suggest diverse functions for the N-ethylmaleimide sensitive factor

Whiteheart, SW, Matveeva, EA

J. Struct. Biol. 2004
19164740 Membrane fusion: grappling with SNARE and SM proteins

Südhof, TC, Rothman, JE

Science 2009
21689688 Requirements for the catalytic cycle of the N-ethylmaleimide-Sensitive Factor (NSF)

Zhao, C, Smith, EC, Whiteheart, SW

Biochim. Biophys. Acta 2012
10559959 An NSF function distinct from ATPase-dependent SNARE disassembly is essential for Golgi membrane fusion

Müller, JM, Rabouille, C, Newman, R, Shorter, J, Freemont, P, Schiavo, G, Warren, G, Shima, DT

Nat. Cell Biol. 1999
12070132 Sequential SNARE disassembly and GATE-16-GOS-28 complex assembly mediated by distinct NSF activities drives Golgi membrane fusion

Muller, JM, Shorter, J, Newman, R, Deinhardt, K, Sagiv, Y, Elazar, Z, Warren, G, Shima, DT

J. Cell Biol. 2002
Participant Of
Catalyst Activity
Catalyst Activity
ATPase activity of STX10:STX16:VTI1A:VAMP3:NSF hexamer:SNAPs [trans-Golgi network membrane]
Physical Entity
Orthologous Events