Pre-snRNA transcript initiation, Integrator binding, LEC binding

Stable Identifier
R-HSA-6814549
Type
Reaction [omitted]
Species
Homo sapiens
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In an unknown order of events, RNA polymerase II initiates transcription and the Integrator complex (Baillat et al. 2005) and Little Elongation Complex (LEC, Hu et al. 2013) are recruited to phosphorylated RNA polymerase II (Egloff et al. 2010). The Integrator complex interacts with RPAP2, which binds phosphoserine-7 of the C-terminal domain (CTD) of RNA polymerase II and is required for recruitment of Integrator (Egloff et al. 2007, Egloff et al. 2012). RPAP2 interacts with the putative scaffold proteins RPRD1A and RPRD1B at the CTD (Ni et al. 2011, Ni et al. 2014) and DSIF is required for recruitment of Integrator (Skaar et al. 2015). The Integrator complex does not seem to play a significant role in subsequent elongation of the pre-snRNA transcript but is critical for processing of the 3' end of the pre-snRNA.

Literature References
PubMed ID Title Journal Year
18079403 Serine-7 of the RNA polymerase II CTD is specifically required for snRNA gene expression

Egloff, S, O'Reilly, D, Chapman, RD, Taylor, A, Tanzhaus, K, Pitts, L, Eick, D, Murphy, S

Science 2007
23932780 The little elongation complex functions at initiation and elongation phases of snRNA gene transcription

Hu, D, Smith, ER, Garruss, AS, Mohaghegh, N, Varberg, JM, Lin, C, Jackson, J, Gao, X, Saraf, A, Florens, LA, Washburn, MP, Eissenberg, JC, Shilatifard, A

Mol. Cell 2013
22137580 Ser7 phosphorylation of the CTD recruits the RPAP2 Ser5 phosphatase to snRNA genes

Egloff, S, Zaborowska, J, Laitem, C, Kiss, T, Murphy, S

Mol. Cell 2012
24997600 RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation

Ni, Z, Xu, C, Guo, X, Hunter, GO, Kuznetsova, OV, Tempel, W, Marcon, E, Zhong, G, Guo, H, Kuo, WH, Li, J, Young, P, Olsen, JB, Wan, C, Loppnau, P, El Bakkouri, M, Senisterra, GA, He, H, Huang, H, Sidhu, SS, Emili, A, Murphy, S, Mosley, AL, Arrowsmith, CH, Min, J, Greenblatt, JF

Nat. Struct. Mol. Biol. 2014
20457598 The integrator complex recognizes a new double mark on the RNA polymerase II carboxyl-terminal domain

Egloff, S, Szczepaniak, SA, Dienstbier, M, Taylor, A, Knight, S, Murphy, S

J. Biol. Chem. 2010
25675981 The Integrator complex controls the termination of transcription at diverse classes of gene targets

Skaar, JR, Ferris, AL, Wu, X, Saraf, A, Khanna, KK, Florens, LA, Washburn, MP, Hughes, SH, Pagano, M

Cell Res. 2015
16239144 Integrator, a multiprotein mediator of small nuclear RNA processing, associates with the C-terminal repeat of RNA polymerase II

Baillat, D, Hakimi, MA, Näär, AM, Shilatifard, A, Cooch, N, Shiekhattar, R

Cell 2005
22231121 Control of the RNA polymerase II phosphorylation state in promoter regions by CTD interaction domain-containing proteins RPRD1A and RPRD1B

Ni, Z, Olsen, JB, Guo, X, Zhong, G, Ruan, ED, Marcon, E, Young, P, Guo, H, Li, J, Moffat, J, Emili, A, Greenblatt, JF

Transcription 2011
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Title
RNA polymerase II activity of RPAP2:RPRD1A,B:RPRD2:RNA polymerase II (phosphoserine-5,7):Initiation factors:snRNA gene [nucleoplasm]
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