Vesicle is tethered through binding GOLGA2:GORASP1, GOLGB1 and the COG complex

Stable Identifier
Homo sapiens
Locations in the PathwayBrowser
SVG |   | PPTX  | SBGN
Click the image above or here to open this reaction in the Pathway Browser
The layout of this reaction may differ from that in the pathway view due to the constraints in pathway layout

Vesicles tethering at the cis-Golgi is mediated both by long coiled-coil tethers and by large multisubunit complexes. Vesicle-bound USO1 homodimers associate with the Golgi localized GOLGA2:GORASP1 complex and with the Golgi-localized octameric COG tethering complex (Allan et al, 2000; Moyer et al, 2001; Weide et al, 2001; Nakamura et al, 1997; Seeman et al, 2000; Sohda et al, 2007). GOLGB1 is another Golgi localized tether that may facilitate vesicle tethering at the cis-Golgi, although it is also implicated in intra-Golgi retrograde trafficking (Linstedt and Hauri, 1993; Sonnichsen et al, 1998; Alvarez et al, 2001; Beard et al, 2005; reviewed in Appenzeller-Herzog et al, 2006). In addition to binding to USO1, the COG complex also interacts with components of the COPI coat, as well as with SNARE proteins and the TMEM115 protein (Suvorova et al, 2002; Zolov et al, 2005; Shestakova et al, 2007; Ong et al, 2014; reviewed in Willet et al, 2013).

Literature References
PubMed ID Title Journal Year
15728195 Cog3p depletion blocks vesicle-mediated Golgi retrograde trafficking in HeLa cells

Lupashin, VV, Zolov, SN

J. Cell Biol. 2005
17274799 The interaction of two tethering factors, p115 and COG complex, is required for Golgi integrity

Ogata, S, Sohda, M, Ikehara, Y, Fusano, T, Sakisaka, S, Nakamura, N, Misumi, Y, Yoshimura, S

Traffic 2007
9150144 The vesicle docking protein p115 binds GM130, a cis-Golgi matrix protein, in a mitotically regulated manner

Warren, G, Levine, TP, Lowe, M, Rabouille, C, Nakamura, N

Cell 1997
11035033 The p115-interactive proteins GM130 and giantin participate in endoplasmic reticulum-Golgi traffic

Hauri, HP, Sztul, E, Alvarez, C, Garcia-Mata, R

J. Biol. Chem. 2001
11306556 The Golgi matrix protein GM130: a specific interacting partner of the small GTPase rab1b

Weide, T, Köster, M, Barnekow, A, Bayer, M, Siebrasse, JP, Peters, R

EMBO Rep. 2001
10903204 Rab1 recruitment of p115 into a cis-SNARE complex: programming budding COPII vesicles for fusion

Allan, BB, Balch, WE, Moyer, BD

Science 2000
23839779 The Golgi puppet master: COG complex at center stage of membrane trafficking interactions

Ungar, D, Lupashin, V, Willett, R

Histochem. Cell Biol. 2013
9490716 A role for giantin in docking COPI vesicles to Golgi membranes

Warren, G, Dirac-Svejstrup, B, Lowe, M, Levine, T, Jämsä, E, Sönnichsen, B

J. Cell Biol. 1998
11285137 Rab1 interaction with a GM130 effector complex regulates COPII vesicle cis--Golgi tethering

Allan, BB, Balch, WE, Moyer, BD

Traffic 2001
7691276 Giantin, a novel conserved Golgi membrane protein containing a cytoplasmic domain of at least 350 kDa

Hauri, HP, Linstedt, AD

Mol. Biol. Cell 1993
16723730 The ER-Golgi intermediate compartment (ERGIC): in search of its identity and function

Hauri, HP, Appenzeller-Herzog, C

J. Cell. Sci. 2006
18086915 Interaction of the conserved oligomeric Golgi complex with t-SNARE Syntaxin5a/Sed5 enhances intra-Golgi SNARE complex stability

Shestakova, A, Suvorova, E, Pavliv, O, Lupashin, V, Khaidakova, G

J. Cell Biol. 2007
24806965 TMEM115 is an integral membrane protein of the Golgi complex involved in retrograde transport

Hong, W, Ong, YS, Gounko, NV, Tran, TH

J. Cell. Sci. 2014
15878873 A cryptic Rab1-binding site in the p115 tethering protein

Warren, G, Satoh, A, Shorter, J, Beard, M

J. Biol. Chem. 2005
10679020 The role of the tethering proteins p115 and GM130 in transport through the Golgi apparatus in vivo

Seemann, J, Jokitalo, EJ, Warren, G

Mol. Biol. Cell 2000
Orthologous Events
Cite Us!