ARFGAP, cargo, v-SNAREs and p24 proteins bind nascent COPI complex

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Reaction [binding]
Homo sapiens
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Binding and polymerization of the coatomer (the COPI coat) is coordinated with the incorporation of cargo proteins and Golgi-targeting snares, as well as with recruitment of ARFGAP proteins (Letourneur et al, 1994; Nagahama et al,1996; Bremser et al, 1999).
Typical model cargo for COPI-mediated trafficking includes the viral glycoprotein VSV-G and proinsulin as well as the KDEL receptors, which bind and recycle ER-resident proteins and which themselves must be returned to post-ER compartments (Cosson and Letourner, 1994; Ben-Tekaya et al, 2005; Majoul et al, 2001; Orci et al, 1997, Bremser et al, 1999; Presley et al, 1997; reviewed in Beck et al, 2009).
Other protein components of the COPI vesicle include the p24 family of proteins, which serve diverse roles in the early secretory pathway (reviewed in Schuiki and Volchuk, 2012). Oligomeric p24 proteins interact with ADP-bound ARF and components of the COPI coat, contributing to coatomer recruitment and oligomerization (Gommel et al, 2001; Majoul et al, 2001; Bethune et al, 2006; Harter and Wieland, 1998; Langer et al, 2008; Reinhard et al, 1999). The p24 proteins also act as cargo receptors for various proteins destined for packaging in COPI vesicles; these include GPI-anchored transmembrane proteins, WNT ligands and some G-protein coupled receptors (Takida et al, 2008; Bonnon et al, 2010; Luo et al, 2011; Beuchling et al, 2011; Wang and Kazanietz, 2002; reviewed in Schuiki and Volchuk, 2012). Finally, the p24 proteins contribute to COPI coat disassembly by restricting ARF GTPase activity until cargo has been loaded (Goldberg, 2000; Lanoix et al, 2001).
ARFGAPs are recruited to the budding vesicle through direct interaction with active ARF, the cytoplasmic tails of cargo proteins and with components of the COPI coat (Goldberg, 2000; Majoul et al, 2001; Aoe et al, 1997; Kliouchnikov et al, 2009; Luo et al, 2009). Stimulation of ARF GTPase activity is coordinated with cargo recruitment to ensure that only cargo-loaded vesicles are produced (Goldberg, 2000; Luo et al, 2009).
Mammalian cells have 3 ARFGAPs that appear to be involved in COPI-mediated traffic, ARFGAP1,2 and 3 (Frigerio et al, 2007; Liu et al, 2001; Kahn et al, 2008). ARFGAP1 has a ALPS domain that recognizes membrane curvature and that is required for the GTPase stimulating activity of the protein, suggesting a mechanism for coordinating ARF1-mediated GTP hydrolysis with vesicle formation (Bigay et al, 2003; Mesmin et al, 2007). ARFGAP 2 and 3 do not contain this motif, and their activity is dependent upon interaction with coatomer (Weimar et al 2008; Kliouchnikov et al, 2009; Luo et al, 2009).
Finally, there is evidence that components of the ankyin/spectrin skeleton may be incorporated in the nascent COPI vesicle, acting as a bridge between cargo proteins and the dynein-dynactin complex required for their transport to the Golgi (Devarajan et al, 1997; Godi et al, 1998; Holleran et al, 1996; Holleran et al, 2001).
Literature References
PubMed ID Title Journal Year
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Hayashi, R, Randazzo, PA, Ha, VL, Luo, R

Cell. Signal. 2009
9380700 Na,K-ATPase transport from endoplasmic reticulum to Golgi requires the Golgi spectrin-ankyrin G119 skeleton in Madin Darby canine kidney cells

Morrow, JS, Stabach, PR, Devarajan, P, De Matteis, MA

Proc. Natl. Acad. Sci. U.S.A. 1997
8001155 Coatomer is essential for retrieval of dilysine-tagged proteins to the endoplasmic reticulum

Riezman, H, Hennecke, S, Emr, SD, Gaynor, EC, Letourneur, F, Cosson, P, Duden, R, Démollière, C

Cell 1994
8128252 Coatomer interaction with di-lysine endoplasmic reticulum retention motifs

Letourneur, F, Cosson, P

Science 1994
19015319 Differential roles of ArfGAP1, ArfGAP2, and ArfGAP3 in COPI trafficking

Weimer, C, Moelleken, J, Wieland, F, Brugger, B, Eckert, P, Beck, R, Reckmann, I

J. Cell Biol. 2008
11689559 Chimaerins, novel non-protein kinase C phorbol ester receptors, associate with Tmp21-I (p23): evidence for a novel anchoring mechanism involving the chimaerin C1 domain

Wang, H, Kazanietz, MG

J. Biol. Chem. 2002
18809720 Consensus nomenclature for the human ArfGAP domain-containing proteins

Zapp, ML, Theibert, AB, Inoue, H, Satake, M, Premont, RT, Logsdon, JM, Randazzo, PA, Bruford, E, Nie, Z, Kahn, RA, Cassel, D

J. Cell Biol. 2008
10052452 Coupling of coat assembly and vesicle budding to packaging of putative cargo receptors

Söllner, TH, Schweikert, M, Bremser, M, Nickel, W, Hughes, CA, Ravazzola, M, Amherdt, M, Wieland, FT, Rothman, JE

Cell 1999
9288971 ER-to-Golgi transport visualized in living cells

Presley, JF, Lippincott-Schwartz, J, Hirschberg, K, Cole, NB, Zaal, KJ, Schroer, TA

Nature 1997
9751720 A single binding site for dilysine retrieval motifs and p23 within the gamma subunit of coatomer

Harter, C, Wieland, FT

Proc. Natl. Acad. Sci. U.S.A. 1998
11461920 beta III spectrin binds to the Arp1 subunit of dynactin

Morrow, JS, Stankewich, MC, Ligon, LA, Tokito, M, Holzbaur, EL, Holleran, EA

J. Biol. Chem. 2001
15632110 Live imaging of bidirectional traffic from the ERGIC

Hauri, HP, Ben-Tekaya, H, Pepperkok, R, Miura, K

J. Cell. Sci. 2005
25436559 Diverse roles for the p24 family of proteins in eukaryotic cells

Schuiki, I, Volchuk, A

Biomol Concepts 2012
10761932 Decoding of sorting signals by coatomer through a GTPase switch in the COPI coat complex

Goldberg, J

Cell 2000
11172815 Functional characterization of novel human ARFGAP3

Zhang, C, Liu, X, Xing, G, Chen, Q, He, F

FEBS Lett. 2001
17253781 Two lipid-packing sensor motifs contribute to the sensitivity of ArfGAP1 to membrane curvature

Bigay, J, Drin, G, Rawet, M, Mesmin, B, Levi, S, Antonny, B, Cassel, D

Biochemistry 2007
11703931 KDEL-cargo regulates interactions between proteins involved in COPI vesicle traffic: measurements in living cells using FRET

Hell, SW, Straub, M, Majoul, I, Söling, HD, Duden, R

Dev. Cell 2001
14654841 Lipid packing sensed by ArfGAP1 couples COPI coat disassembly to membrane bilayer curvature

Gounon, P, Antonny, B, Bigay, J, Robineau, S

Nature 2003
17760859 Two human ARFGAPs associated with COP-I-coated vesicles

Frigerio, G, Majoul, I, Grimsey, N, Dale, M, Duden, R

Traffic 2007
17927562 Mammalian GPI-anchored proteins require p24 proteins for their efficient transport from the ER to the plasma membrane

Kinoshita, T, Maeda, Y, Takida, S

Biochem. J. 2008
16940185 Coatomer, the coat protein of COPI transport vesicles, discriminates endoplasmic reticulum residents from p24 proteins

Wieland, F, Brugger, B, Hoffmann, J, Reckmann, I, Kol, M, Béthune, J

Mol. Cell. Biol. 2006
9990005 Receptor-induced polymerization of coatomer

Bremser, M, Wieland, F, Sohn, K, Brugger, B, Reinhard, C, Harter, C, Helms, JB

Proc. Natl. Acad. Sci. U.S.A. 1999
22094269 p24 proteins are required for secretion of Wnt ligands

Boutros, M, Weiss, M, Buechling, T, Spirohn, K, Chaudhary, V

EMBO Rep. 2011
9405360 The KDEL receptor, ERD2, regulates intracellular traffic by recruiting a GTPase-activating protein for ARF1

Aoe, T, Cukierman, E, Peters, PJ, Lee, A, Hsu, VW, Cassel, D

EMBO J. 1997
21219331 Proteinase-activated receptors, nucleotide P2Y receptors, and μ-opioid receptor-1B are under the control of the type I transmembrane proteins p23 and p24A in post-Golgi trafficking

Wang, Y, Luo, W, Reiser, G

J. Neurochem. 2011
16633337 ARF proteins: roles in membrane traffic and beyond

Chavrier, P, D'Souza-Schorey, C

Nat. Rev. Mol. Cell Biol. 2006
18182008 A conformational change in the alpha-subunit of coatomer induced by ligand binding to gamma-COP revealed by single-pair FRET

Brugger, B, Wieland, FT, Herten, DP, Stoops, EH, Béthune, J, Roth, CM, Langer, JD

Traffic 2008
20427317 Selective export of human GPI-anchored proteins from the endoplasmic reticulum

Hauri, HP, Wendeler, MW, Bonnon, C, Paccaud, JP

J. Cell. Sci. 2010
9671725 ADP ribosylation factor regulates spectrin binding to the Golgi complex

Godi, A, Di Tullio, G, Morrow, JS, Santone, I, Stabach, PR, Luini, A, Polishchuk, R, Devarajan, P, Pertile, P, De Matteis, MA, Petrucci, TC

Proc. Natl. Acad. Sci. U.S.A. 1998
11726511 Recruitment to Golgi membranes of ADP-ribosylation factor 1 is mediated by the cytoplasmic domain of p23

Lottspeich, F, Nickel, W, Reinhard, C, Wieland, FT, Helms, JB, Pfannstiel, J, Heiss, A, Memon, AR, Lechner, J, Gommel, DU

EMBO J. 2001
8991093 Centractin (ARP1) associates with spectrin revealing a potential mechanism to link dynactin to intracellular organelles

Karki, S, Holzbaur, EL, Tokito, MK, Holleran, EA

J. Cell Biol. 1996
19631211 The COPI system: molecular mechanisms and function

Rawet, M, Ravet, M, Wieland, FT, Beck, R, Cassel, D

FEBS Lett. 2009
9244307 Bidirectional transport by distinct populations of COPI-coated vesicles

Söllner, TH, Perrelet, A, Ravazzola, M, Stamnes, M, Orci, L, Amherdt, M, Rothman, JE

Cell 1997
11748249 Sorting of Golgi resident proteins into different subpopulations of COPI vesicles: a role for ArfGAP1

Lanoix, J, Stark, A, Szafer, E, Weiss, M, Dejgaard, K, Nilsson, T, Cassel, D, Ouwendijk, J

J. Cell Biol. 2001
8636227 A v-SNARE implicated in intra-Golgi transport

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J. Cell Biol. 1996
19109418 Discrete determinants in ArfGAP2/3 conferring Golgi localization and regulation by the COPI coat

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Mol. Biol. Cell 2009
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