RAB1:GTP recruits GBF1 to the ERGIC

Stable Identifier
Reaction [binding]
Homo sapiens
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In its GTP-bound active state, RAB1 recruits the ARF GEF GBF1 to the ERGIC (Monetta et al, 2007). GBF1 is the only ARF activator required for the formation of COPI coats, and it therefore has roles in the anterograde ERGIC-to-cis-Golgi pathway as well as in COPI-mediated retrograde transport within the Golgi and back to the ERGIC and ER (Kawamoto et al, 2002; Szul et al, 2005; Zhao et al, 2006; Szul et al, 2007; reviewed in Szul and Sztul, 2011). GBF1 activates ARF4 which is concentrated at the ERGIC compartment, but also ARF1 and ARF5 which have more generalized localization within the secretory pathway (Volpicelli-Daley et al, 2005; Chun et al, 2008; reviewed in D'Souza-Schorey and Chavrier, 2006). GBF1 also interacts with the USO1 homodimer, a long coiled-coil tethering factor (Garcia-Mata and Sztul, 2003).

Literature References
PubMed ID Title Journal Year
12634853 The membrane-tethering protein p115 interacts with GBF1, an ARF guanine-nucleotide-exchange factor

Sztul, E, Garcia-Mata, R

EMBO Rep. 2003
12047556 GBF1, a guanine nucleotide exchange factor for ADP-ribosylation factors, is localized to the cis-Golgi and involved in membrane association of the COPI coat

Yoshida, Y, Tamaki, H, Kawamoto, K, Yamashina, S, Nakayama, K, Torii, S, Shinotsuka, C

Traffic 2002
18524849 Characterization of class I and II ADP-ribosylation factors (Arfs) in live cells: GDP-bound class II Arfs associate with the ER-Golgi intermediate compartment independently of GBF1

Presley, JF, Melançon, P, Chun, J, Dejgaard, SY, Shapovalova, Z

Mol. Biol. Cell 2008
22013193 COPII and COPI traffic at the ER-Golgi interface

Sztul, E, Szul, T

Physiology (Bethesda) 2011
17429068 Rab1b interacts with GBF1 and modulates both ARF1 dynamics and COPI association

Slavin, I, Monetta, P, Romero, N, Alvarez, C

Mol. Biol. Cell 2007
16030262 Isoform-selective effects of the depletion of ADP-ribosylation factors 1-5 on membrane traffic

Volpicelli-Daley, LA, Zhang, CJ, Li, Y, Kahn, RA

Mol. Biol. Cell 2005
15813748 Dissection of membrane dynamics of the ARF-guanine nucleotide exchange factor GBF1

Shestopal, S, Sztul, E, Szul, T, Alvarez, C, Brandon, E, Garcia-Mata, R

Traffic 2005
16926190 GBF1, a cis-Golgi and VTCs-localized ARF-GEF, is implicated in ER-to-Golgi protein traffic

Presley, JF, Melançon, P, Claude, A, Zhao, X, Chun, J, Shields, DJ

J. Cell. Sci. 2006
16633337 ARF proteins: roles in membrane traffic and beyond

Chavrier, P, D'Souza-Schorey, C

Nat. Rev. Mol. Cell Biol. 2006
17956946 Dissecting the role of the ARF guanine nucleotide exchange factor GBF1 in Golgi biogenesis and protein trafficking

Grabski, R, Shestopal, S, Morohashi, Y, Lyons, S, Sztul, E, Szul, T, Lowe, M

J. Cell. Sci. 2007
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