HC23,45-HMGB1:LPS [extracellular region]

Stable Identifier
R-HSA-6801229
Type
Complex
Species
Homo sapiens
Compartment
Locations in the PathwayBrowser
General

Analysis of LPS binding to HMGB1 was conducted using a surface plasmon resonance analyses (BIAcore) and ELISA test with LPS-immobilized microtiter wells (Youn JH et al. 2008). Pull-down assays using streptavidin agarose beads and ELISA assay were conducted to map regions within the A and B box domains of HMGB1 that bind to the polysaccharide and lipid A moieties of LPS respectively (Youn JH et al. 2011).

Literature References
PubMed ID Title Journal Year
18354232 High mobility group box 1 protein binding to lipopolysaccharide facilitates transfer of lipopolysaccharide to CD14 and enhances lipopolysaccharide-mediated TNF-alpha production in human monocytes

Youn, JH, Oh, YJ, Kim, ES, Choi, JE, Shin, JS

J. Immunol. 2008
21660935 Identification of lipopolysaccharide-binding peptide regions within HMGB1 and their effects on subclinical endotoxemia in a mouse model

Youn, JH, Kwak, MS, Wu, J, Kim, ES, Ji, Y, Min, HJ, Yoo, JH, Choi, JE, Cho, HS, Shin, JS

Eur. J. Immunol. 2011
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