Reactome | HC23,45-HMGB1:LPS [extracellular region]

HC23,45-HMGB1:LPS [extracellular region]

Stable Identifier

R-HSA-6801229

Type

Complex

Species

Homo sapiens

Compartment

extracellular region

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General

Analysis of LPS binding to HMGB1 was conducted using a surface plasmon resonance analyses (BIAcore) and ELISA test with LPS-immobilized microtiter wells (Youn JH et al. 2008). Pull-down assays using streptavidin agarose beads and ELISA assay were conducted to map regions within the A and B box domains of HMGB1 that bind to the polysaccharide and lipid A moieties of LPS respectively (Youn JH et al. 2011).

Literature References

PubMed ID	Title	Journal	Year
21660935	Identification of lipopolysaccharide-binding peptide regions within HMGB1 and their effects on subclinical endotoxemia in a mouse model Choi, JE, Wu, J, Youn, JH, Min, HJ, Shin, JS, Ji, Y, Kwak, MS, Kim, ES, Yoo, JH, Cho, HS	Eur. J. Immunol.	2011
18354232	High mobility group box 1 protein binding to lipopolysaccharide facilitates transfer of lipopolysaccharide to CD14 and enhances lipopolysaccharide-mediated TNF-alpha production in human monocytes Choi, JE, Youn, JH, Shin, JS, Kim, ES, Oh, YJ	J. Immunol.	2008