SUMOylated XPC is recognized by the SUMO-targeted ubiquitin ligase RNF111 (Arcadia) that, together with the E2 ubiquitin conjugating complex of UBE2N (UBC13) and UBE2V2 (MMS2), generates K63-linked polyubiquitin chains on XPC (Poulsen et al. 2013) to efficiently release XPC from UV lesions (van Cuijk et al. 2015). The release of K63-polyubiquitinated XPC occurs from GG-NER pre-incision complexes that contain TFIIH and XPA and promotes optimal access/binding of ERCC5 (XPG) endonuclease to the pre-incision complex (van Cuijk et al. 2015). Successful binding of ERCC5 endonuclease 3' to the damage facilitates binding of the ERCC1:ERCC4 (ERCC1:XPF) endonuclease and progression of the NER reaction.
Poulsen, SL, Hansen, RK, Wagner, SA, van Cuijk, L, van Belle, GJ, Streicher, W, Wikström, M, Choudhary, C, Houtsmuller, AB, Marteijn, JA, Bekker-Jensen, S, Mailand, N
van Cuijk, L, van Belle, GJ, Turkyilmaz, Y, Poulsen, SL, Janssens, RC, Theil, AF, Sabatella, M, Lans, H, Mailand, N, Houtsmuller, AB, Vermeulen, W, Marteijn, JA
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