SUMOylated XPC is recognized by the SUMO-targeted ubiquitin ligase RNF111 (Arcadia) that, together with the E2 ubiquitin conjugating complex of UBE2N (UBC13) and UBE2V2 (MMS2), generates K63-linked polyubiquitin chains on XPC (Poulsen et al. 2013) to efficiently release XPC from UV lesions (van Cuijk et al. 2015). The release of K63-polyubiquitinated XPC occurs from GG-NER pre-incision complexes that contain TFIIH and XPA and promotes optimal access/binding of ERCC5 (XPG) endonuclease to the pre-incision complex (van Cuijk et al. 2015). Successful binding of ERCC5 endonuclease 3' to the damage facilitates binding of the ERCC1:ERCC4 (ERCC1:XPF) endonuclease and progression of the NER reaction.
Houtsmuller, AB, Lans, H, Sabatella, M, Poulsen, SL, Theil, AF, Janssens, RC, van Belle, GJ, Marteijn, JA, van Cuijk, L, Turkyilmaz, Y, Mailand, N, Vermeulen, W
Houtsmuller, AB, Poulsen, SL, Hansen, RK, Wikström, M, van Belle, GJ, Marteijn, JA, van Cuijk, L, Streicher, W, Choudhary, C, Bekker-Jensen, S, Mailand, N, Wagner, SA
ubiquitin protein ligase activity of RNF111 [nucleoplasm]
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