TSTA3 dimer reduces GDP-KDGal to GDP-Fuc

Stable Identifier
R-HSA-6787642
Type
Reaction [transition]
Species
Homo sapiens
Compartment
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The de novo synthesis pathway for GDP-L-fucose is a two step pathway starting from GDP-mannose. In the second step, GDP-4-dehydro-6-deoxy-alpha-D-mannose (GDP-DHDMan) is epimerised and reduced to GDP-L-fucose (GDP-Fuc). The cytosolic enzyme GDP-L-fucose synthase (TSTA3, aka FX) appears to have both epimerase and reductase activities and functions as a homodimer (Tonetti et al. 1996, Zhou et al. 2013). In the second stage, 4-reductase activity of TSTA3 dimer catalyses a hydride transfer from NADPH to the keto group at C-4 of GDP-4-keto-6-deoxygalactose (GDP-KDGal), yielding GDP-fucose (GDP-Fuc) and NADP+.

Literature References
PubMed ID Title Journal Year
8910301 Synthesis of GDP-L-fucose by the human FX protein

Tonetti, M, Sturla, L, Bisso, A, Benatti, U, De Flora, A

J. Biol. Chem. 1996
23774504 The crystal structure of human GDP-L-fucose synthase

Zhou, H, Sun, L, Li, J, Xu, C, Yu, F, Liu, Y, Ji, C, He, J

Acta Biochim. Biophys. Sin. (Shanghai) 2013
Participants
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Catalyst Activity
Title
GDP-L-fucose synthase activity of TSTA3 dimer [cytosol]
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Activity
Orthologous Events
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