TSTA3 dimer reduces GDP-KDGal to GDP-Fuc

Stable Identifier
R-HSA-6787642
Type
Reaction
Species
Homo sapiens
Compartment
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The de novo synthesis pathway for GDP-L-fucose is a two step pathway starting from GDP-mannose. In the second step, GDP-4-dehydro-6-deoxy-alpha-D-mannose (GDP-DHDMan) is epimerised and reduced to GDP-L-fucose (GDP-Fuc). The cytosolic enzyme GDP-L-fucose synthase (TSTA3, aka FX) appears to have both epimerase and reductase activities and functions as a homodimer (Tonetti et al. 1996, Zhou et al. 2013). In the second stage, 4-reductase activity of TSTA3 dimer catalyses a hydride transfer from NADPH to the keto group at C-4 of GDP-4-keto-6-deoxygalactose (GDP-KDGal), yielding GDP-fucose (GDP-Fuc) and NADP+.

Literature References
PubMed ID Title Journal Year
23774504 The crystal structure of human GDP-L-fucose synthase

Yu, F, Liu, Y, Xu, C, Sun, L, Ji, C, He, J, Li, J, Zhou, H

Acta Biochim. Biophys. Sin. (Shanghai) 2013
8910301 Synthesis of GDP-L-fucose by the human FX protein

Bisso, A, De Flora, A, Benatti, U, Tonetti, M, Sturla, L

J. Biol. Chem. 1996
Participants
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Catalyst Activity

GDP-L-fucose synthase activity of TSTA3 dimer [cytosol]

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