Reactome | TSTA3 dimer reduces GDP-KDGal to GDP-Fuc

TSTA3 dimer reduces GDP-KDGal to GDP-Fuc

Stable Identifier

R-HSA-6787642

Type

Reaction [transition]

Species

Homo sapiens

Compartment

cytosol

ReviewStatus

5/5

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Metabolism of proteins (Homo sapiens)

- Post-translational protein modification (Homo sapiens)
  - Asparagine N-linked glycosylation (Homo sapiens)
    - Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein (Homo sapiens)
      - Synthesis of substrates in N-glycan biosythesis (Homo sapiens)
        
        GDP-fucose biosynthesis (Homo sapiens)
        
        TSTA3 dimer reduces GDP-KDGal to GDP-Fuc (Homo sapiens)

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TSTA3 dimer reduces GDP-KDGal to GDP-Fuc

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The de novo synthesis pathway for GDP-L-fucose is a two step pathway starting from GDP-mannose. In the second step, GDP-4-dehydro-6-deoxy-alpha-D-mannose (GDP-DHDMan) is epimerised and reduced to GDP-L-fucose (GDP-Fuc). The cytosolic enzyme GDP-L-fucose synthase (TSTA3, aka FX) appears to have both epimerase and reductase activities and functions as a homodimer (Tonetti et al. 1996, Zhou et al. 2013). In the second stage, 4-reductase activity of TSTA3 dimer catalyses a hydride transfer from NADPH to the keto group at C-4 of GDP-4-keto-6-deoxygalactose (GDP-KDGal), yielding GDP-fucose (GDP-Fuc) and NADP+.

Literature References

PubMed ID	Title	Journal	Year
23774504	The crystal structure of human GDP-L-fucose synthase Yu, F, Liu, Y, Xu, C, Sun, L, Ji, C, He, J, Li, J, Zhou, H	Acta Biochim. Biophys. Sin. (Shanghai)	2013
8910301	Synthesis of GDP-L-fucose by the human FX protein Bisso, A, De Flora, A, Benatti, U, Tonetti, M, Sturla, L	J. Biol. Chem.	1996