CDKAL1:4Fe-4S methylthiolates N6-threonylcarbamoyladenosine-37 in tRNA yielding 2-methylthio-N6-threonylcarbamoyladenosine-37

Stable Identifier
R-HSA-6786571
Type
Reaction [BlackBoxEvent]
Species
Homo sapiens
Compartment
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The CDKAL1:4Fe-4S complex methylthiolates N6-threonylcarbamoyladenosine-37 in several tRNAs (Arragain et al. 2010). The source of the methyl group is S-adenosylmethionine (AdoMet). The source of the sulfur is unknown. The homologue in Bacillus subtilis, mtaB, catalyzes the same reaction (Arragain et al. 2010).
CDKAL1 is located on the cytosolic face of the endoplasmic reticulum therefore the reaction is presumed to occur in the cytosol (Brambillasca et al. 2012).

Literature References
PubMed ID Title Journal Year
20584901 Identification of eukaryotic and prokaryotic methylthiotransferase for biosynthesis of 2-methylthio-N6-threonylcarbamoyladenosine in tRNA

Arragain, S, Handelman, SK, Forouhar, F, Wei, FY, Tomizawa, K, Hunt, JF, Douki, T, Fontecave, M, Mulliez, E, Atta, M

J. Biol. Chem. 2010
23048041 CDK5 regulatory subunit-associated protein 1-like 1 (CDKAL1) is a tail-anchored protein in the endoplasmic reticulum (ER) of insulinoma cells

Brambillasca, S, Altkrueger, A, Colombo, SF, Friederich, A, Eickelmann, P, Mark, M, Borgese, N, Solimena, M

J. Biol. Chem. 2012
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Catalyst Activity
Title
N6-threonylcarbomyladenosine methylthiotransferase activity of CDKAL1:4Fe-4S [endoplasmic reticulum membrane]
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