ALKBH8 methylates 5-carboxymethyluridine-34 in tRNA(Arg) and tRNA(Glu) yielding 5-methoxycarbonylmethyluridine-34

Stable Identifier
R-HSA-6786500
Type
Reaction [transition]
Species
Homo sapiens
Compartment
cytosol
ReviewStatus
5/5
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ALKBH8 methylates 5-carboxymethyluridine-34 in tRNA(Arg) and tRNA(Glu) yielding 5-methoxycarbonylmethyluridine-34
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ALKBH8:Fe2+ transfers a methyl group from S-adenosylmethionine (AdoMet) to 5-carboxymethyluridine-34 of tRNA, yielding 5-methoxycarbonylmethyluridine-34 (5-(2-methoxy-2-oxoethyl)uridine-34) (Fu et al. 2010, Songe-Møller et al. 2010). The corresponding homologue in Saccharomyces, Trm9p, catalyzes the same reaction.
Literature References
PubMed ID Title Journal Year
20308323 Human AlkB homolog ABH8 Is a tRNA methyltransferase required for wobble uridine modification and DNA damage survival

Dedon, PC, Chan, CT, Brophy, JA, Paules, RS, Atmore, KA, Fu, D, Samson, LD, Begley, U, Begley, TJ

Mol. Cell. Biol. 2010
20123966 Mammalian ALKBH8 possesses tRNA methyltransferase activity required for the biogenesis of multiple wobble uridine modifications implicated in translational decoding

van den Born, E, Falnes, PØ, Kirpekar, F, Songe-Møller, L, Vågbø, CB, Kristoffersen, T, Leihne, V, Krokan, HE, Klungland, A

Mol. Cell. Biol. 2010
Participants
Input
Output
Participates
as an event of
Event Information
Go Biological Process
tRNA modification (0006400)
Catalyst Activity

tRNA methyltransferase activity of ALKBH8:Fe2+ [cytosol]

Physical Entity
ALKBH8:Fe2+ [cytosol] (Homo sapiens)
Activity
tRNA methyltransferase activity (GO:0008175)
Inferred From
TRM9 methylates 5-carboxymethyluridine-34 in tRNA(Arg3) yielding 5-methoxycarbonylmethyluridine-34 (Saccharomyces cerevisiae)
Authored
May, B  (2015-06-30)
Reviewed
Levinger, L  (2015-08-11)
Motorin, Y  (2015-08-25)
Jarrous, N  (2015-10-24)
Created
May, B  (2015-07-09)
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