ALKBH8 methylates 5-carboxymethyluridine-34 in tRNA(Arg) and tRNA(Glu) yielding 5-methoxycarbonylmethyluridine-34

Stable Identifier
R-HSA-6786500
Type
Reaction [transition]
Species
Homo sapiens
Compartment
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ALKBH8:Fe2+ transfers a methyl group from S-adenosylmethionine (AdoMet) to 5-carboxymethyluridine-34 of tRNA, yielding 5-methoxycarbonylmethyluridine-34 (5-(2-methoxy-2-oxoethyl)uridine-34) (Fu et al. 2010, Songe-Møller et al. 2010). The corresponding homologue in Saccharomyces, Trm9p, catalyzes the same reaction.

Literature References
PubMed ID Title Journal Year
20123966 Mammalian ALKBH8 possesses tRNA methyltransferase activity required for the biogenesis of multiple wobble uridine modifications implicated in translational decoding

Songe-Møller, L, van den Born, E, Leihne, V, Vågbø, CB, Kristoffersen, T, Krokan, HE, Kirpekar, F, Falnes, PØ, Klungland, A

Mol. Cell. Biol. 2010
20308323 Human AlkB homolog ABH8 Is a tRNA methyltransferase required for wobble uridine modification and DNA damage survival

Fu, D, Brophy, JA, Chan, CT, Atmore, KA, Begley, U, Paules, RS, Dedon, PC, Begley, TJ, Samson, LD

Mol. Cell. Biol. 2010
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Catalyst Activity
Catalyst Activity
Title
tRNA methyltransferase activity of ALKBH8:Fe2+ [cytosol]
Physical Entity
Activity
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