ALKBH8 methylates 5-carboxymethyluridine-34 in tRNA(Arg) and tRNA(Glu) yielding 5-methoxycarbonylmethyluridine-34

Stable Identifier
Reaction [transition]
Homo sapiens
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ALKBH8:Fe2+ transfers a methyl group from S-adenosylmethionine (AdoMet) to 5-carboxymethyluridine-34 of tRNA, yielding 5-methoxycarbonylmethyluridine-34 (5-(2-methoxy-2-oxoethyl)uridine-34) (Fu et al. 2010, Songe-Møller et al. 2010). The corresponding homologue in Saccharomyces, Trm9p, catalyzes the same reaction.

Literature References
PubMed ID Title Journal Year
20308323 Human AlkB homolog ABH8 Is a tRNA methyltransferase required for wobble uridine modification and DNA damage survival

Dedon, PC, Chan, CT, Brophy, JA, Paules, RS, Atmore, KA, Fu, D, Samson, LD, Begley, U, Begley, TJ

Mol. Cell. Biol. 2010
20123966 Mammalian ALKBH8 possesses tRNA methyltransferase activity required for the biogenesis of multiple wobble uridine modifications implicated in translational decoding

van den Born, E, Falnes, PØ, Kirpekar, F, Songe-Møller, L, Vågbø, CB, Kristoffersen, T, Leihne, V, Krokan, HE, Klungland, A

Mol. Cell. Biol. 2010
Event Information
Go Biological Process
Catalyst Activity

tRNA methyltransferase activity of ALKBH8:Fe2+ [cytosol]

Inferred From
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