TRIT1 transfers dimethylallyl group to adenosine-37 of tRNAs

Stable Identifier
R-HSA-6784462
Type
Reaction [transition]
Species
Homo sapiens
Compartment
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TRIT1 transfers a dimethylallyl group (isopentenyl group) from dimethylallyl diphosphate to the N6 position of adenosine-37 in tRNA(Ser), yielding N6-dimethylallyladenosine-37 (N6-isopentenyladenosine-37) (Golovko et al. 2000, Spinola et al. 2005, Lamichhane et al. 2013, Yarham et al. 2014, Smaldino et al. 2015). TRIT1 modifies both cytosolic and mitochondrial tRNAs and a mutation in TRIT1 causes mitochondrial respiratory defects (Yarham et al. 2014). Expression of TRIT1 is down-regulated in lung adenocarcinomas compared with normal tissue (Spinola et al. 2005). The homologue in Saccharomyces cerevisiae, MOD5, catalyzes the same reaction.

Literature References
PubMed ID Title Journal Year
24901367 Defective i6A37 modification of mitochondrial and cytosolic tRNAs results from pathogenic mutations in TRIT1 and its substrate tRNA

Yarham, JW, Lamichhane, TN, Pyle, A, Mattijssen, S, Baruffini, E, Bruni, F, Donnini, C, Vassilev, A, He, L, Blakely, EL, Griffin, H, Santibanez-Koref, M, Bindoff, LA, Ferrero, I, Chinnery, PF, McFarland, R, Maraia, RJ, Taylor, RW

PLoS Genet. 2014
15870694 Identification and functional characterization of the candidate tumor suppressor gene TRIT1 in human lung cancer

Spinola, M, Galvan, A, Pignatiello, C, Conti, B, Pastorino, U, Nicander, B, Paroni, R, Dragani, TA

Oncogene 2005
25261850 The cytoplasmic and nuclear populations of the eukaryote tRNA-isopentenyl transferase have distinct functions with implications in human cancer

Smaldino, PJ, Read, DF, Pratt-Hyatt, M, Hopper, AK, Engelke, DR

Gene 2015
11111046 Cloning of a human tRNA isopentenyl transferase

Golovko, A, Hjälm, G, Sitbon, F, Nicander, B

Gene 2000
24126054 Human cells have a limited set of tRNA anticodon loop substrates of the tRNA isopentenyltransferase TRIT1 tumor suppressor

Lamichhane, TN, Mattijssen, S, Maraia, RJ

Mol. Cell. Biol. 2013
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Title
tRNA dimethylallyltransferase activity of TRIT1 [cytosol]
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