3' incision by ERCC5 (XPG) in TC-NER

Stable Identifier
R-HSA-6782224
Type
Reaction [transition]
Species
Homo sapiens
Compartment
nucleoplasm
ReviewStatus
5/5
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3' incision by ERCC5 (XPG) in TC-NER
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In transcription-coupled nucleotide excision repair (TC-NER), as well as in global genome nucleotide excision repair (GG-NER), the cleavage of the damaged DNA strand 3' to the site of damage is carried out by a DNA endonuclease XPG (ERCC5). While the NER-mediated DNA synthesis may be initiated prior to the 3' incision (Staresincic et al. 2009), the components of the incision complex probably dissociate from the NER site shortly after the DNA synthesis complex assembly and 3' incision (Overmeer et al. 2011). The exception is the RPA heterotrimer, which is a constituent of the NER post-incision complex, and also coats the undamaged DNA strand, thereby protecting it from endonucleolytic cleavage. RNA polymerase II-associated factors also remain bound to the TC-NER site.
Literature References
PubMed ID Title Journal Year
21282463 Replication protein A safeguards genome integrity by controlling NER incision events

Fousteri, M, Mullenders, LH, Volker, M, Moser, J, Kool, H, van Zeeland, AA, Overmeer, RM, Tomkinson, AE

J. Cell Biol. 2011
19279666 Coordination of dual incision and repair synthesis in human nucleotide excision repair

Wijgers, N, Staresincic, L, Schärer, OD, Gourdin, AM, Fagbemi, AF, Enzlin, JH, Dunand-Sauthier, I, Clarkson, SG, Giglia-Mari, G, Vermeulen, W

EMBO J. 2009
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Output
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Catalyst Activity

DNA endonuclease activity of TC-NER incision complex: 5'-incised damaged DNA:trimmed nascent mRNA:(PCNA:POLD,POLE), (MonoUb:K164-PCNA:POLK):RPA:RFC [nucleoplasm]

Physical Entity
TC-NER incision complex: 5'-incised damaged DNA:trimmed nascent mRNA:(PCNA:POLD,POLE), (MonoUb:K164-PCNA:POLK):RPA:RFC [nucleoplasm] (Homo sapiens)
Activity
DNA endonuclease activity (GO:0004520)
Orthologous Events
3' incision by ERCC5 (XPG) in TC-NER (Bos taurus)
3' incision by ERCC5 (XPG) in TC-NER (Canis familiaris)
3' incision by ERCC5 (XPG) in TC-NER (Mus musculus)
3' incision by ERCC5 (XPG) in TC-NER (Rattus norvegicus)
3' incision by ERCC5 (XPG) in TC-NER (Schizosaccharomyces pombe)
3' incision by ERCC5 (XPG) in TC-NER (Sus scrofa)
Authored
Orlic-Milacic, M  (2015-06-16)
Hoeijmakers, JH  (2004-01-29)
Reviewed
Fousteri, M  (2015-08-03)
Created
Orlic-Milacic, M  (2015-06-05)
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