Active RNA Pol II complex transcribes lesion-containing DNA template

Stable Identifier
R-HSA-6781824
Type
Reaction [BlackBoxEvent]
Species
Homo sapiens
Compartment
nucleoplasm
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Active RNA Pol II complex transcribes lesion-containing DNA template
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An active RNA polymerase II complex (RNA Pol II, POLR2) transcribes a damaged DNA template. Once damaged DNA bases, such as cyclobutane pyrimidine dimers (CPDs), enter the active site of the polymerase, RNA Pol II misincorporates a ribonucleotide into nascent mRNA, which blocks the translocation step and results in polymerase stalling. In the stalled complex, the lesion is inaccessible, while the RNA Pol II conformation is unchanged (Brueckner et al. 2007).

Literature References
PubMed ID Title Journal Year
17290000 CPD damage recognition by transcribing RNA polymerase II

Carell, T, Brueckner, F, Cramer, P, Hennecke, U

Science 2007
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Catalyst Activity

DNA-directed 5'-3' RNA polymerase activity of Damaged dsDNA with open transcription bubble:Hyperphosphorylated RNA Pol II:TFIIH [nucleoplasm]

Physical Entity
Damaged dsDNA with open transcription bubble:Hyperphosphorylated RNA Pol II:TFIIH [nucleoplasm] (Homo sapiens)
Activity
DNA-directed 5'-3' RNA polymerase activity (GO:0003899)
Orthologous Events
Active RNA Pol II complex transcribes lesion-containing DNA template (Caenorhabditis elegans)
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Active RNA Pol II complex transcribes lesion-containing DNA template (Dictyostelium discoideum)
Active RNA Pol II complex transcribes lesion-containing DNA template (Drosophila melanogaster)
Active RNA Pol II complex transcribes lesion-containing DNA template (Gallus gallus)
Active RNA Pol II complex transcribes lesion-containing DNA template (Mus musculus)
Active RNA Pol II complex transcribes lesion-containing DNA template (Plasmodium falciparum)
Active RNA Pol II complex transcribes lesion-containing DNA template (Rattus norvegicus)
Active RNA Pol II complex transcribes lesion-containing DNA template (Saccharomyces cerevisiae)
Active RNA Pol II complex transcribes lesion-containing DNA template (Schizosaccharomyces pombe)
Active RNA Pol II complex transcribes lesion-containing DNA template (Sus scrofa)
Authored
Orlic-Milacic, M  (2015-06-16)
Gopinathrao, G  (2004-02-02)
Hoeijmakers, JH  (2004-01-29)
Reviewed
Fousteri, M  (2015-08-03)
Created
Orlic-Milacic, M  (2015-06-02)
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