Reactome | ELAC2 cleaves the 3' end of pre-tRNA

ELAC2 cleaves the 3' end of pre-tRNA

Stable Identifier

R-HSA-5696815

Type

Reaction [transition]

Species

Homo sapiens

Compartment

nucleoplasm

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ELAC2, a RNaseZ activity in humans, endonucleolytically cleaves pre-tRNAs near the 3' end, generating a 3' hydroxyl group to which the nucleotides CCA will be polymerized (Takaku et al. 2004, Yan et al. 2006, Elbarbary et al. 2008). ELAC2 is located in both the nucleus and mitochondria (Brzezniak et al. 2011). ELAC1, a homologue of ELAC2, is located in the cytosol (Rossmanith 2011) and cleaves 3' ends of tRNAs in vitro (Takaku et al. 2003).

Literature References

PubMed ID	Title	Journal	Year
18809514	Functional analyses for tRNase Z variants: an aspartate and a histidine in the active site are essential for the catalytic activity Elbarbary, RA, Takaku, H, Nashimoto, M	Biochim. Biophys. Acta	2008
21593607	Involvement of human ELAC2 gene product in 3' end processing of mitochondrial tRNAs Bijata, M, Brzezniak, LK, Stepien, PP, Szczesny, RJ	RNA Biol	2011
15317868	The N-terminal half-domain of the long form of tRNase Z is required for the RNase 65 activity Minagawa, A, Takaku, H, Nashimoto, M, Takagi, M	Nucleic Acids Res.	2004
21559454	Localization of human RNase Z isoforms: dual nuclear/mitochondrial targeting of the ELAC2 gene product by alternative translation initiation Rossmanith, W	PLoS ONE	2011
12711671	A candidate prostate cancer susceptibility gene encodes tRNA 3' processing endoribonuclease Minagawa, A, Takaku, H, Nashimoto, M, Takagi, M	Nucleic Acids Res.	2003
16361254	Naturally occurring mutations in human mitochondrial pre-tRNASer(UCN) can affect the transfer ribonuclease Z cleavage site, processing kinetics, and substrate secondary structure Zareen, N, Levinger, L, Yan, H	J. Biol. Chem.	2006

Participants

Input

5' cleaved pre-tRNA [nucleoplasm] (Homo sapiens)

Output

5',3' cleaved pre-tRNA [nucleoplasm] (Homo sapiens)

Participates

as an event of

tRNA processing in the nucleus (Homo sapiens)

Event Information

Go Biological Process

tRNA 3'-end processing (0042780)

Catalyst Activity

tRNA-specific ribonuclease activity of ELAC2 [nucleoplasm]

Physical Entity

ELAC2 [nucleoplasm] (Homo sapiens)

Activity

tRNA-specific ribonuclease activity (GO:0004549)

Authored

May, B (2015-05-29)

Reviewed

Levinger, L (2015-08-11)

Motorin, Y (2015-08-25)

Jarrous, N (2015-10-24)

Created

May, B (2015-05-30)