RNase P cleaves the 5' end of pre-tRNA

Stable Identifier
Reaction [transition]
Homo sapiens
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The RNase P RNA-protein complex located in the nucleus endonucleolytically cleaves near the 5' end of pre-tRNAs, generating the mature 5' end (Ferrari et al. 1980, Bartkiewicz et al. 1989, Jiang et al. 2001, Reiner et al. 2011, reviewed in Jarrous 2002). The site of cleavage is determined by the length of the helices in the acceptor and T stems of the tRNA (Yuan and Altman 1995). Human cells contain distinct nuclear and mitochondrial RNase P activities (Rossmanith et al. 1995), with nuclear RNase P localized in the nucleolus (Jarrous et al. 1999). The nuclear RNase P is similar to bacterial enzymes in having a catalytic RNA component. The mitochondrial RNase P is unusual in containing only protein subunits (Holzmann et al. 2008).
Literature References
PubMed ID Title Journal Year
10444065 Localization in the nucleolus and coiled bodies of protein subunits of the ribonucleoprotein ribonuclease P

Lee, C, Altman, S, Wolenski, JS, Jarrous, N, Wesolowski, D

J. Cell Biol. 1999
7828588 Substrate recognition by human RNase P: identification of small, model substrates for the enzyme

Yuan, Y, Altman, S

EMBO J. 1995
18984158 RNase P without RNA: identification and functional reconstitution of the human mitochondrial tRNA processing enzyme

Bennett, KL, Löffler, E, Rossmanith, W, Gerner, C, Holzmann, J, Frank, P

Cell 2008
11871657 Human ribonuclease P: subunits, function, and intranuclear localization

Jarrous, N

RNA 2002
11455963 Protein-RNA interactions in the subunits of human nuclear RNase P

Altman, S, Jiang, T, Guerrier-Takada, C

RNA 2001
7759547 Human mitochondrial tRNA processing

Rossmanith, W, Potuschak, T, Karwan, R, Tullo, A, Sbisà, E

J. Biol. Chem. 1995
6930639 Specific RNA-cleaving activities from HeLa cells

Dickson, E, Yehle, CO, Ferrari, S, Robertson, HD

Proc. Natl. Acad. Sci. U.S.A. 1980
2470644 Identification and characterization of an RNA molecule that copurifies with RNase P activity from HeLa cells

Bartkiewicz, M, Gold, H, Altman, S

Genes Dev. 1989
21450806 RNA binding properties of conserved protein subunits of human RNase P

Alfiya-Mor, N, Wesolowski, D, Jarrous, N, Reiner, R, Altman, S, Berrebi-Demma, M

Nucleic Acids Res. 2011
Event Information
Go Biological Process
Catalyst Activity

ribonuclease P activity of RNase P [nucleoplasm]

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