TRNT1 polymerizes CCA at the 3' end of pre-tRNA

Stable Identifier
R-HSA-5696807
Type
Reaction [transition]
Species
Homo sapiens
Compartment
nucleoplasm
ReviewStatus
5/5
Locations in the PathwayBrowser
Expand all
General
SVG | 
PNG
Low
Medium
High
 | PPTX  | SBGN
TRNT1 polymerizes CCA at the 3' end of pre-tRNA
Click the image above or here to open this reaction in the Pathway Browser
The layout of this reaction may differ from that in the pathway view due to the constraints in pathway layout
TRNT1 (CCA-adding enzyme) polymerizes the non-templated nucleotides CCA onto the 3' end (acceptor arm) of tRNAs (Reichert et al. 2001, Lizano et al. 2007, Lizano et al. 2008). In contrast to some eubacterial tRNA genes, eukaryotic tRNA genes do not encode CCA at the 3' end. The added CCA sequence is determined by the structural properties of the TRNT1 protein itself rather than by any nucleic acid template (Augustin et al. 2003, Ernst et al. 2015, Kuhn et al. 2015, see also the structure of the bacterial enzyme in Li et al. 2002). After polymerization of a single CCA motif, conformational change of the enzyme ejects the tRNA before additional nucleotides can be polymerized (Kuhn et al. 2015). Aberrant RNA with two CCA motifs is targeted for destruction.
Literature References
PubMed ID Title Journal Year
12729736 Crystal structure of the human CCA-adding enzyme: insights into template-independent polymerization

Augustin, MA, Reichert, AS, Betat, H, Huber, R, Mörl, M, Steegborn, C

J. Mol. Biol. 2003
18226598 A comparative analysis of CCA-adding enzymes from human and E. coli: differences in CCA addition and tRNA 3'-end repair

Lizano, E, Scheibe, M, Rammelt, C, Betat, H, Mörl, M

Biochimie 2008
11727826 A eubacterial origin for the human tRNA nucleotidyltransferase?

Reichert, AS, Thurlow, DL, Mörl, M

Biol. Chem. 2001
17204286 A splice variant of the human CCA-adding enzyme with modified activity

Lizano, E, Schuster, J, Muller, M, Kelso, J, Mörl, M

J. Mol. Biol. 2007
12526808 Crystal structures of the Bacillus stearothermophilus CCA-adding enzyme and its complexes with ATP or CTP

Li, F, Xiong, Y, Wang, J, Cho, HD, Tomita, K, Weiner, AM, Steitz, TA

Cell 2002
25849199 Domain movements during CCA-addition: A new function for motif C in the catalytic core of the human tRNA nucleotidyltransferases

Ernst, FG, Rickert, C, Bluschke, A, Betat, H, Steinhoff, HJ, Mörl, M

RNA Biol 2015
25640237 On-enzyme refolding permits small RNA and tRNA surveillance by the CCA-adding enzyme

Kuhn, CD, Wilusz, JE, Zheng, Y, Beal, PA, Joshua-Tor, L

Cell 2015
Participants
Input
Output
Participates
as an event of
Event Information
Go Biological Process
tRNA 3'-end processing (0042780)
Catalyst Activity

5'-3' RNA polymerase activity of TRNT1 [nucleoplasm]

Physical Entity
TRNT1 [nucleoplasm] (Homo sapiens)
Activity
5'-3' RNA polymerase activity (GO:0034062)
Authored
May, B  (2015-05-29)
Reviewed
Levinger, L  (2015-08-11)
Motorin, Y  (2015-08-25)
Jarrous, N  (2015-10-24)
Created
May, B  (2015-05-30)
Cite Us!