ASRGL1 hydrolyses aspartame to L-Asp, L-Phe

Stable Identifier
R-HSA-5696365
Type
Reaction [transition]
Species
Homo sapiens
Compartment
cytosol
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ASRGL1 hydrolyses aspartame to L-Asp, L-Phe
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The human isoaspartyl peptidase/L-asparaginase (ASRGL1) is an N-terminal nucleophile (Ntn) hydrolase superfamily member that catalyses the hydrolysis of l-asparagine and beta-aspartyl-dipeptides and their methyl esters such as aspartame (beta-L-Asp-L-Phe methyl ester) (Cantor et al. 2009, Li et al. 2012, Nomme et al. 2014). ASRGL1 is a cytosolic enzyme that is active as a heterodimer of alpha and beta chains, formed by autocleavage between Gly167 and Thr168 (Nomme et al. 2012). ASRGL1 is expressed in brain, kidney, testis and the gastrointestinal tract. Aspartame is an artificial sweetner used as a sugar substitute in some drinks. Sufferers of phenylketonuria (PKU) are advised to avoid aspartame as one of its breakdown products, phenylalanine, could contribute to the excess pool of phenylalanine that PKU sufferers cannot metabolise from the body.

Literature References
PubMed ID Title Journal Year
22861376 Structures of apo and product-bound human L-asparaginase: insights into the mechanism of autoproteolysis and substrate hydrolysis

Nomme, J, Su, Y, Konrad, M, Lavie, A

Biochemistry 2012
22891768 Uncoupling intramolecular processing and substrate hydrolysis in the N-terminal nucleophile hydrolase hASRGL1 by circular permutation

Li, W, Cantor, JR, Yogesha, SD, Yang, S, Chantranupong, L, Liu, JQ, Agnello, G, Georgiou, G, Stone, EM, Zhang, Y

ACS Chem. Biol. 2012
24768817 Elucidation of the specific function of the conserved threonine triad responsible for human L-asparaginase autocleavage and substrate hydrolysis

Nomme, J, Su, Y, Lavie, A

J. Mol. Biol. 2014
19839645 The human asparaginase-like protein 1 hASRGL1 is an Ntn hydrolase with beta-aspartyl peptidase activity

Cantor, JR, Stone, EM, Chantranupong, L, Georgiou, G

Biochemistry 2009
Participants
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Output
Participant Of
hasEvent
Catalyst Activity
Catalyst Activity
Title
beta-aspartyl-peptidase activity of ASRGL1 heterodimer [cytosol]
Physical Entity
ASRGL1 heterodimer [cytosol]
Activity
beta-aspartyl-peptidase activity (0008798)
Orthologous Events
ASRGL1 hydrolyses aspartame to L-Asp, L-Phe (Bos taurus)
ASRGL1 hydrolyses aspartame to L-Asp, L-Phe (Canis familiaris)
ASRGL1 hydrolyses aspartame to L-Asp, L-Phe (Danio rerio)
ASRGL1 hydrolyses aspartame to L-Asp, L-Phe (Drosophila melanogaster)
ASRGL1 hydrolyses aspartame to L-Asp, L-Phe (Gallus gallus)
ASRGL1 hydrolyses aspartame to L-Asp, L-Phe (Mus musculus)
ASRGL1 hydrolyses aspartame to L-Asp, L-Phe (Rattus norvegicus)
ASRGL1 hydrolyses aspartame to L-Asp, L-Phe (Sus scrofa)
ASRGL1 hydrolyses aspartame to L-Asp, L-Phe (Xenopus tropicalis)
Authored
Jassal, B  (2015-05-28)
Reviewed
D'Eustachio, P  (2015-06-26)
Created
Jassal, B  (2015-05-28)