BET1:GOSR2:STX5 bind v-SNARES on tethered vesicle

Stable Identifier
Reaction [dissociation]
Homo sapiens
Locations in the PathwayBrowser
SVG |   | PPTX  | SBGN
Click the image above or here to open this reaction in the Pathway Browser
The layout of this reaction may differ from that in the pathway view due to the constraints in pathway layout

After displacement of the COPII coat, a tetrameric SNAREpin forms between the v-SNARE and the three t-SNARE proteins (Hay et al, 1997; Hay et al, 1998; Hui et al, 1997; Bentley et al, 2006; reviewed in Hong and Lev 2014). This interaction is promoted by the tethering factor USO1 and by the STX5-interacting protein SCFD1 (Wang et al, 2014; Dascher and Balch, 1996; Cao and Barlowe 2000; Flanagan and Barlowe, 2006; reviewed in Lord et al, 2013). The zippering of the SNAREpin provides the mechanical force that drives membrane fusion, releasing cargo into the acceptor compartment (reviewed in Sudhof and Rothman, 2009).

Literature References
PubMed ID Title Journal Year
16303754 Cysteine-disulfide cross-linking to monitor SNARE complex assembly during endoplasmic reticulum-Golgi transport

Flanagan, JJ, Barlowe, C

J. Biol. Chem. 2006
9647643 Localization, dynamics, and protein interactions reveal distinct roles for ER and Golgi SNAREs

Steegmaier, M, Klumperman, J, Oorschot, V, Kuo, CS, Scheller, RH, Hay, JC

J. Cell Biol. 1998
24119662 Tethering the assembly of SNARE complexes

Hong, W, Lev, S

Trends Cell Biol. 2014
8663406 Mammalian Sly1 regulates syntaxin 5 function in endoplasmic reticulum to Golgi transport

Balch, WE, Dascher, C

J. Biol. Chem. 1996
25406594 p115-SNARE interactions: a dynamic cycle of p115 binding monomeric SNARE motifs and releasing assembled bundles

Grabski, R, Wang, T, Sztul, E, Hay, JC

Traffic 2015
19164740 Membrane fusion: grappling with SNARE and SM proteins

Südhof, TC, Rothman, JE

Science 2009
23378591 The highly conserved COPII coat complex sorts cargo from the endoplasmic reticulum and targets it to the golgi

Ferro-Novick, S, Miller, EA, Lord, C

Cold Spring Harb Perspect Biol 2013
9307973 An isoform of the Golgi t-SNARE, syntaxin 5, with an endoplasmic reticulum retrieval signal

Nakamura, N, Nilsson, T, Shima, DT, Hui, N, Warren, G, Sönnichsen, B

Mol. Biol. Cell 1997
9094723 Protein interactions regulating vesicle transport between the endoplasmic reticulum and Golgi apparatus in mammalian cells

Kuo, CS, Scheller, RH, Chao, DS, Hay, JC

Cell 1997
10747087 Asymmetric requirements for a Rab GTPase and SNARE proteins in fusion of COPII vesicles with acceptor membranes

Cao, X, Barlowe, C

J. Cell Biol. 2000
17038314 SNARE status regulates tether recruitment and function in homotypic COPII vesicle fusion

Bentley, M, Xu, D, Liang, Y, Mullen, K, Sztul, E, Hay, JC

J. Biol. Chem. 2006
Orthologous Events
Cite Us!