Reactome | Initial resection of double-strand break ends

Initial resection of double-strand break ends

Stable Identifier

R-HSA-5693608

Type

Reaction [transition]

Species

Homo sapiens

Compartment

nucleoplasm

ReviewStatus

5/5

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Initial resection of double-strand break ends

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In order for repair of the DNA double-strand breaks to occur through homologous recombination or single strand annealing, the 5' ends of the break must first be resected to produce 3' overhanging single stranded DNA (ssDNA) that can subsequently invade homologous duplex DNA (e.g. in the sister chromatid) (Thompson et al. 2001, Kolpashchikov et al. 2001). The MRE11A component of the MRN complex possesses endonuclease activity (Trujillo et al. 1998, Hopfner et al. 2002) that is activated by binding of RBBP8 (CtIP) and BRCA1, in the presence of Mn2+ or Mg2+ ions (Sartori et al. 2007, Yun and Hiom 2009).

Literature References

PubMed ID	Title	Journal	Year
11376695	Homologous recombinational repair of DNA ensures mammalian chromosome stability Thompson, LH, Schild, D	Mutat Res	2001
11139606	Polarity of human replication protein A binding to DNA. Wold, MS, Favre, A, Lavrik, OI, Khodyreva, SN, Khlimankov, DY, Kolpashchikov, DM	Nucleic Acids Res	2001
9705271	Nuclease activities in a complex of human recombination and DNA repair factors Rad50, Mre11, and p95. Lee, EY, Trujillo, KM, Yuan, SS, Sung, P	J Biol Chem	1998
17965729	Human CtIP promotes DNA end resection Sartori, AA, Lukas, C, Jackson, SP, Baer, R, Mistrik, M, Lukas, J, Bartek, J, Fu, S, Coates, J	Nature	2007
19357644	CtIP-BRCA1 modulates the choice of DNA double-strand-break repair pathway throughout the cell cycle Yun, MH, Hiom, K	Nature	2009
12152085	The Rad50 zinc-hook is a structure joining Mre11 complexes in DNA recombination and repair. Tainer, JA, Carney, JP, Bodmer, JL, McMurray, CT, Karcher, A, Henderson, B, Usui, T, Owen, BA, Moncalian, G, Zinkel, RA, Hopfner, KP, Craig, L, Petrini, JH	Nature	2002