Reactome | ATM recognizes H2AFX-Nucleosomes

ATM recognizes H2AFX-Nucleosomes

Stable Identifier

R-HSA-5693602

Type

Reaction [binding]

Species

Homo sapiens

Compartment

nucleoplasm

ReviewStatus

5/5

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H2AFX (also known as H2AX) is a variant of histone H2A and is present in a portion of nucleosomes. While H2AFX-containing nucleosomes (H2AFX-nucleosomes) are not specifically recruited to the sites of DNA double-strand breaks (DSBs), ATM recognizes the carboxyl tails of H2AFX on H2AFX-nucleosomes in the vicinity of DSBs as a suitable phosphorylation substrate. The phosphorylated H2AFX (gamma-H2AX) plays a crucial role in the retention of DNA repair proteins at DSBs, manifested in the formation of ionizing radiation-induced foci (Paull et al. 2000, Celeste et al. 2002, Redon et al. 2002, Celeste et al. 2003, Fernandez-Capetillo et al. 2004).

Literature References

PubMed ID	Title	Journal	Year
11893489	Histone H2A variants H2AX and H2AZ Redon, C, Pilch, D, Rogakou, E, Sedelnikova, O, Newrock, K, Bonner, W	Curr. Opin. Genet. Dev.	2002
11934988	Genomic instability in mice lacking histone H2AX Celeste, A, Petersen, S, Romanienko, PJ, Fernandez-Capetillo, O, Chen, HT, Sedelnikova, OA, Reina-San-Martin, B, Coppola, V, Meffre, E, Difilippantonio, MJ, Redon, C, Pilch, DR, Olaru, A, Eckhaus, M, Camerini-Otero, RD, Tessarollo, L, Livak, F, Manova, K, Bonner, WM, Nussenzweig, MC, Nussenzweig, A	Science	2002
10959836	A critical role for histone H2AX in recruitment of repair factors to nuclear foci after DNA damage. Paull, TT, Rogakou, EP, Yamazaki, V, Kirchgessner, CU, Gellert, M, Bonner, WM	Curr Biol	2000
12792649	Histone H2AX phosphorylation is dispensable for the initial recognition of DNA breaks. Celeste, A, Fernandez-Capetillo, O, Kruhlak, MJ, Pilch, DR, Staudt, DW, Lee, A, Bonner, RF, Bonner, WM, Nussenzweig, A	Nat Cell Biol	2003
15279782	H2AX: the histone guardian of the genome Fernandez-Capetillo, O, Lee, A, Nussenzweig, M, Nussenzweig, A	DNA Repair (Amst.)	2004