Cleavage of Holliday junctions by GEN1 or SLX1A:SLX4:MUS81:EME1,(MUS81:EME2)

Stable Identifier
R-HSA-5693584
Type
Reaction [transition]
Species
Homo sapiens
Compartment
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Holliday junctions are efficiently cleaved either by GEN1 endonuclease or the SLX-MUS complex, composed of the SLX1A:SLX4 heterodimer and the heterodimer of MUS81 and EME1 (or, possibly, EME2). Both SLX1A:SLX4 and MUS1:EME1,EME2 possess endonucleolytic activity and act in a coordinated fashion. SLX1A:SLX4 cleaves the double Holliday junction first, which is followed by MUS81:EME1 (or MUS81:EME2) mediated cleavage of the incised Holliday junction. Cleavage by both GEN1 and SLX-MUS predominantly leads to exchange of genetic material between sister chromatids, creating crossover products (Wyatt et al. 2013, Sarbajna et al. 2014).

Literature References
PubMed ID Title Journal Year
24831703 Roles of SLX1-SLX4, MUS81-EME1, and GEN1 in avoiding genome instability and mitotic catastrophe

Sarbajna, S, Davies, D, West, SC

Genes Dev. 2014
24076221 Coordinated actions of SLX1-SLX4 and MUS81-EME1 for Holliday junction resolution in human cells

Wyatt, HD, Sarbajna, S, Matos, J, West, SC

Mol. Cell 2013
Participants
Participant Of
Catalyst Activity
Catalyst Activity
Title
endodeoxyribonuclease activity of SLX1A:SLX4:MUS81:EME1,(MUS81:EME2),GEN1 [nucleoplasm]
Physical Entity
Activity
Orthologous Events
Authored
Reviewed
Created