Cleavage of Holliday junctions by GEN1 or SLX1A:SLX4:MUS81:EME1,(MUS81:EME2)

Stable Identifier
R-HSA-5693584
Type
Reaction [transition]
Species
Homo sapiens
Compartment
nucleoplasm
ReviewStatus
5/5
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Cleavage of Holliday junctions by GEN1 or SLX1A:SLX4:MUS81:EME1,(MUS81:EME2)
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Holliday junctions are efficiently cleaved either by GEN1 endonuclease or the SLX-MUS complex, composed of the SLX1A:SLX4 heterodimer and the heterodimer of MUS81 and EME1 (or, possibly, EME2). Both SLX1A:SLX4 and MUS1:EME1,EME2 possess endonucleolytic activity and act in a coordinated fashion. SLX1A:SLX4 cleaves the double Holliday junction first, which is followed by MUS81:EME1 (or MUS81:EME2) mediated cleavage of the incised Holliday junction. Cleavage by both GEN1 and SLX-MUS predominantly leads to exchange of genetic material between sister chromatids, creating crossover products (Wyatt et al. 2013, Sarbajna et al. 2014).
Literature References
PubMed ID Title Journal Year
24076221 Coordinated actions of SLX1-SLX4 and MUS81-EME1 for Holliday junction resolution in human cells

West, SC, Matos, J, Sarbajna, S, Wyatt, HD

Mol. Cell 2013
24831703 Roles of SLX1-SLX4, MUS81-EME1, and GEN1 in avoiding genome instability and mitotic catastrophe

West, SC, Sarbajna, S, Davies, D

Genes Dev. 2014
Participants
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Output
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as an event of
Catalyst Activity

DNA endonuclease activity of SLX1A:SLX4:MUS81:EME1,(MUS81:EME2),GEN1 [nucleoplasm]

Physical Entity
SLX1A:SLX4:MUS81:EME1,(MUS81:EME2),GEN1 [nucleoplasm] (Homo sapiens)
Activity
DNA endonuclease activity (GO:0004520)
Orthologous Events
Cleavage of Holliday junctions by GEN1 or SLX1A:SLX4:MUS81:EME1,(MUS81:EME2) (Bos taurus)
Cleavage of Holliday junctions by GEN1 or SLX1A:SLX4:MUS81:EME1,(MUS81:EME2) (Canis familiaris)
Cleavage of Holliday junctions by GEN1 or SLX1A:SLX4:MUS81:EME1,(MUS81:EME2) (Gallus gallus)
Cleavage of Holliday junctions by GEN1 or SLX1A:SLX4:MUS81:EME1,(MUS81:EME2) (Mus musculus)
Cleavage of Holliday junctions by GEN1 or SLX1A:SLX4:MUS81:EME1,(MUS81:EME2) (Rattus norvegicus)
Cleavage of Holliday junctions by GEN1 or SLX1A:SLX4:MUS81:EME1,(MUS81:EME2) (Sus scrofa)
Authored
Orlic-Milacic, M  (2015-05-12)
Matthews, L  (2003-09-10)
Reviewed
Borowiec, JA  (2015-06-12)
Created
Orlic-Milacic, M  (2015-05-16)
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