Resolution of D-loop Structures through Synthesis-Dependent Strand Annealing (SDSA)

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Homo sapiens
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In the synthesis-dependent strand-annealing (SDSA) model of D-loop resolution, D-loop strands extended by DNA repair synthesis dissociate from their sister chromatid complements and reanneal with their original complementary strands, resulting in non-crossover products (Mitchel et al. 2010). SDSA is promoted by the DNA helicase RTEL1 (Barber et al. 2008, Uringa et al. 2012). Additional DNA synthesis occurs to fill the remaining single strand gap present in the reannealed DNA duplex. DNA polymerase alpha has been implicated in this late step of DNA repair synthesis (Levy et al. 2009), although RTEL1-mediated recruitment of PCNA-bound DNA polymerases may also be involved (Vannier et al. 2013). The remaining single strand nicks are closed by DNA ligases, possibly LIG1 or LIG3 (Mortusewicz et al. 2006, Puebla-Osorio et al. 2006).
Literature References
PubMed ID Title Journal Year
18957201 RTEL1 maintains genomic stability by suppressing homologous recombination

West, SC, Collis, SJ, Barber, LJ, Cantor, SB, McIlwraith, MJ, Rose, AM, Martin, JS, Auclair, M, Ward, JD, Tissenbaum, H, O'Neil, NJ, Petalcorin, MI, Youds, JL, Boulton, SJ

Cell 2008
24115439 RTEL1 is a replisome-associated helicase that promotes telomere and genome-wide replication

Ding, H, Petalcorin, MI, Sandhu, S, Boulton, SJ, Wu, X, Vannier, JB, Nabi, Z

Science 2013
16648486 Early embryonic lethality due to targeted inactivation of DNA ligase III

Zhu, C, Alt, FW, Puebla-Osorio, N, Lacey, DB

Mol. Cell. Biol. 2006
19305001 XRCC1 interacts with the p58 subunit of DNA Pol alpha-primase and may coordinate DNA repair and replication during S phase

Bresson, A, Maiorano, D, de Murcia, G, Van Dorsselaer, A, Oehlmann, M, Ménissier-de Murcia, J, Schreiber, V, Delalande, F, Nasheuer, HP, Lévy, N

Nucleic Acids Res. 2009
16855289 Differential recruitment of DNA Ligase I and III to DNA repair sites

Rothbauer, U, Mortusewicz, O, Leonhardt, H, Cardoso, MC

Nucleic Acids Res. 2006
20417600 Molecular structures of crossover and noncrossover intermediates during gap repair in yeast: implications for recombination

Welz-Voegele, C, Jinks-Robertson, S, Zhang, H, Mitchel, K

Mol. Cell 2010
22593209 RTEL1 contributes to DNA replication and repair and telomere maintenance

Zelensky, A, Essers, J, Pickett, HA, Lisaingo, K, Uringa, EJ, Lansdorp, PM, Brind'Amour, J, Rohde, JH

Mol. Biol. Cell 2012
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