Reactome | 3'-incision of DNA by ERCC5 (XPG) in GG-NER

3'-incision of DNA by ERCC5 (XPG) in GG-NER

Stable Identifier

R-HSA-5690988

Type

Reaction [transition]

Species

Homo sapiens

Compartment

nucleoplasm

ReviewStatus

5/5

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3'-incision of DNA by ERCC5 (XPG) in GG-NER

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In global genome nucleotide excision repair (GG-NER), as well as transcription-coupled nucleotide excision repair (TC-NER), the cleavage of the damaged DNA strand 3' to the site of damage is carried out by a DNA endonuclease XPG (ERCC5). While the DNA repair synthesis may be initiated prior to the 3' incision (Staresincic et al. 2009), the components of the incision complex probably dissociate from the NER site shortly after the replicative complex assembly and 3' incision (Overmeer et al. 2011). The exception is the RPA heterotrimer, which is a constituent of the DNA synthesis complex, and also coats the undamaged DNA strand, thereby protecting it from endonucleolytic cleavage.

Literature References

PubMed ID	Title	Journal	Year
21282463	Replication protein A safeguards genome integrity by controlling NER incision events Fousteri, M, Mullenders, LH, Volker, M, Moser, J, Kool, H, van Zeeland, AA, Overmeer, RM, Tomkinson, AE	J. Cell Biol.	2011
19279666	Coordination of dual incision and repair synthesis in human nucleotide excision repair Wijgers, N, Staresincic, L, Schärer, OD, Gourdin, AM, Fagbemi, AF, Enzlin, JH, Dunand-Sauthier, I, Clarkson, SG, Giglia-Mari, G, Vermeulen, W	EMBO J.	2009