Reactome | PPT1 hydrolyses palmitoylated proteins

PPT1 hydrolyses palmitoylated proteins

Stable Identifier

R-HSA-5690517

Type

Reaction [transition]

Species

Homo sapiens

Compartment

lysosomal lumen

Synonyms

H2O + S-hexadecanoyl-L-cysteinyl-(protein) => H(+) + hexadecanoate + L-cysteinyl-(protein)

ReviewStatus

5/5

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The maintenance/regulation of cellular levels of free fatty acids and fatty acyl-CoAs (the activated form of free fatty acids) is extremely important, as imbalances in lipid metabolism can have serious consequences for human health. Free fatty acids can act as detergents to disrupt membranes so their generation is normally tightly regulated to states where they will be rapidly consumed or sequestered. Acyl-coenzyme A thioesterases (ACOTs) hydrolyse the thioester bond in medium- to long-chain fatty acyl-CoAs (of C12-C18 lengths) (MCFAcylCoA, LCFAcylCoA) to their free fatty acids (MCFA, LCFA) (Cohen 2013, Hunt et al. 2012, Kirkby et al. 2010). Lysosomal thioesterase PPT1 is able to specifically hydrolyse palmitic acid (PALM) from palmitoylated proteins (PALM:protein) (Camp & Hofmann 1993, Camp et al. 1994).

Literature References

PubMed ID	Title	Journal	Year
20470824	Functional and structural properties of mammalian acyl-coenzyme A thioesterases Forwood, JK, Kobe, B, Roman, N, Kirkby, B, Kellie, S	Prog. Lipid Res.	2010
7916016	Molecular cloning and expression of palmitoyl-protein thioesterase Camp, LA, Hofmann, SL, Slaughter, CA, Verkruyse, LA, Afendis, SJ	J. Biol. Chem.	1994
7901201	Purification and properties of a palmitoyl-protein thioesterase that cleaves palmitate from H-Ras Camp, LA, Hofmann, SL	J. Biol. Chem.	1993
22465940	The emerging role of acyl-CoA thioesterases and acyltransferases in regulating peroxisomal lipid metabolism Alexson, SE, Siponen, MI, Hunt, MC	Biochim. Biophys. Acta	2012
23700546	New players on the metabolic stage: How do you like Them Acots? Cohen, DE	Adipocyte	2013