PPT1 hydrolyses palmitoylated proteins

Stable Identifier
Reaction [transition]
Homo sapiens
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The maintenance/regulation of cellular levels of free fatty acids and fatty acyl-CoAs (the activated form of free fatty acids) is extremely important, as imbalances in lipid metabolism can have serious consequences for human health. Free fatty acids can act as detergents to disrupt membranes so their generation is normally tightly regulated to states where they will be rapidly consumed or sequestered. Acyl-coenzyme A thioesterases (ACOTs) hydrolyse the thioester bond in medium- to long-chain fatty acyl-CoAs (of C12-C18 lengths) (MCFAcylCoA, LCFAcylCoA) to their free fatty acids (MCFA, LCFA) (Cohen 2013, Hunt et al. 2012, Kirkby et al. 2010). Lysosomal thioesterase PPT1 is able to specifically hydrolyse palmitic acid (PALM) from palmitoylated proteins (PALM:protein) (Camp & Hofmann 1993, Camp et al. 1994).
Literature References
PubMed ID Title Journal Year
20470824 Functional and structural properties of mammalian acyl-coenzyme A thioesterases

Forwood, JK, Kobe, B, Roman, N, Kirkby, B, Kellie, S

Prog. Lipid Res. 2010
7916016 Molecular cloning and expression of palmitoyl-protein thioesterase

Camp, LA, Hofmann, SL, Slaughter, CA, Verkruyse, LA, Afendis, SJ

J. Biol. Chem. 1994
7901201 Purification and properties of a palmitoyl-protein thioesterase that cleaves palmitate from H-Ras

Camp, LA, Hofmann, SL

J. Biol. Chem. 1993
22465940 The emerging role of acyl-CoA thioesterases and acyltransferases in regulating peroxisomal lipid metabolism

Alexson, SE, Siponen, MI, Hunt, MC

Biochim. Biophys. Acta 2012
23700546 New players on the metabolic stage: How do you like Them Acots?

Cohen, DE

Adipocyte 2013
Catalyst Activity

palmitoyl-(protein) hydrolase activity of PPT1 [lysosomal lumen]

Orthologous Events
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