POLQ extends annealed 3'-ssDNA overhangs in MMEJ

Stable Identifier
R-HSA-5687640
Type
Reaction [transition]
Species
Homo sapiens
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DNA polymerase theta (POLQ) extends annealed microhomologous 3'-ssDNA overhangs at DNA double strand breaks (DSBs), using opposing overhangs as templates. POLQ can perform strand displacement synthesis, extending the overhangs beyond ssDNA-dsDNA junction point, which leads to the formation of displaced strand flaps (Kent et al. 2015). PARP1 (or possibly PARP2) is necessary for the recruitment of POLQ to DNA DSBs. POLQ-mediated DNA synthesis during microhomology mediated end joining (MMEJ) (also known as alternative nonhomologous end joining or alt-NHEJ) counteracts homologous recombination repair (HRR) and promotes survival of cells with a compromised HR pathway (Mateos-Gomez et al. 2015). POLQ is error-prone and introduces single nucleotide substitutions during DNA synthesis. HRR-deficient epithelial ovarian cancers frequently overexpress POLQ, which correlates with an increased frequency of somatic point mutations in these tumors (Ceccaldi et al. 2015).

Literature References
PubMed ID Title Journal Year
25642960 Mammalian polymerase ? promotes alternative NHEJ and suppresses recombination

Mateos-Gomez, PA, Gong, F, Nair, N, Miller, KM, Lazzerini-Denchi, E, Sfeir, A

Nature 2015
25643323 Mechanism of microhomology-mediated end-joining promoted by human DNA polymerase ?

Kent, T, Chandramouly, G, McDevitt, SM, Ozdemir, AY, Pomerantz, RT

Nat. Struct. Mol. Biol. 2015
25642963 Homologous-recombination-deficient tumours are dependent on Pol?-mediated repair

Ceccaldi, R, Liu, JC, Amunugama, R, Hajdu, I, Primack, B, Petalcorin, MI, O'Connor, KW, Konstantinopoulos, PA, Elledge, SJ, Boulton, SJ, Yusufzai, T, D'Andrea, AD

Nature 2015
Participants
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Catalyst Activity
Catalyst Activity
Title
DNA-directed DNA polymerase activity of Annealed microhomologous 3'-ssDNA overhangs-DSB:MRN:RBBP8:PARP1,PARP2:FEN1:POLQ [nucleoplasm]
Physical Entity
Activity
Orthologous Events
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Created