PRKDC phosphorylates DCLRE1C at DNA DSBs

Stable Identifier
R-HSA-5687183
Type
Reaction [transition]
Species
Homo sapiens
Compartment
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PRKDC (DNA-PKcs) phosphorylates DCLRE1C (ARTEMIS) at least on serine residue S516, and this phosphorylation is necessary for the activation of DCLRE1C endonucleolytic activity (Ma et al. 2002, Ma et al. 2005, Soubeyrand et al. 2006) as it relieves an autoinhibitory conformation of DCLRE1C (Niewolik et al. 2006).

Literature References
PubMed ID Title Journal Year
16914548 DNA-PKcs dependence of Artemis endonucleolytic activity, differences between hairpins and 5' or 3' overhangs

Kulesza, P, Schwarz, K, Lieber, MR, Lu, H, Wang, LC, Pannicke, U, Zandi, E, Niewolik, D, Ma, Y

J. Biol. Chem. 2006
16600297 Artemis phosphorylated by DNA-dependent protein kinase associates preferentially with discrete regions of chromatin

Soubeyrand, S, Dong, F, de Chasseval, R, Pope, L, Haché, RJ, Gosselin, D, de Villartay, JP

J. Mol. Biol. 2006
11955432 Hairpin opening and overhang processing by an Artemis/DNA-dependent protein kinase complex in nonhomologous end joining and V(D)J recombination.

Schwarz, K, Pannicke, U, Lieber, MR, Ma, Y

Cell 2002
16093244 The DNA-dependent protein kinase catalytic subunit phosphorylation sites in human Artemis

Lieber, MR, Schwarz, K, Lu, H, Pannicke, U, Niewolik, D, Ma, Y

J. Biol. Chem. 2005
Participants
Participates
Catalyst Activity

protein serine/threonine kinase activity of p-T2609,S2612,T2638,T2647-PRKDC:XRCC5:XRCC6:p-S645-DCLRE1C:DNA DSB ends [nucleoplasm]

Orthologous Events
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