ERCC1:XPF cleaves flaps generated by SSA

Stable Identifier
R-HSA-5686657
Type
Reaction [transition]
Species
Homo sapiens
Compartment
nucleoplasm
ReviewStatus
4/5
Locations in the PathwayBrowser
Expand all
General
SVG | 
PNG
Low
Medium
High
 | PPTX  | SBGN
ERCC1:XPF cleaves flaps generated by SSA
Click the image above or here to open this reaction in the Pathway Browser
The layout of this reaction may differ from that in the pathway view due to the constraints in pathway layout
The endonuclease complex ERCC1:XPF (ERCC1:ERCC4) is recruited to single strand annealing (SSA) sites of DNA double-strand break (DSB) repair through direct interaction between XPF (ERCC4) and RAD52 (Motycka et al. 2004). ERCC1:XPF cleaves the ssDNA flaps generated by displacement of non-complementary 3' parts of 3' ssDNA overhangs during RAD52-mediated annealing. The enzymatic activity of ERCC1:XPF is necessary for the completion of SSA (Motycka et al. 2004, Al-Minawi et al. 2008, Ahmad et al. 2008).
Literature References
PubMed ID Title Journal Year
18541667 ERCC1-XPF endonuclease facilitates DNA double-strand break repair

Ahmad, A, Robinson, AR, Duensing, A, van Drunen, E, Beverloo, HB, Weisberg, DB, Hasty, P, Hoeijmakers, JH, Niedernhofer, LJ

Mol. Cell. Biol. 2008
17962301 The ERCC1/XPF endonuclease is required for efficient single-strand annealing and gene conversion in mammalian cells

Al-Minawi, AZ, Saleh-Gohari, N, Helleday, T

Nucleic Acids Res. 2008
14734547 Physical and functional interaction between the XPF/ERCC1 endonuclease and hRad52

Motycka, TA, Bessho, T, Post, SM, Sung, P, Tomkinson, AE

J. Biol. Chem. 2004
Participants
Input
Output
Participates
as an event of
Catalyst Activity

DNA endonuclease activity of ERCC1:ERCC4:RAD51:p-Y104-RAD52:p-RPA:ATR:ATRIP:DNA DSBs with annealed 3' ssDNA overhangs and displaced flaps:p-MRN:p-S1981,Ac-K3016-ATM:KAT5:BRCA1-C complex:EXO1,DNA2:BLM,WRN:p-S990,Ac-K1249-BRIP1:RAD17:RFC:RAD9:HUS1:RAD1:RHNO1:TOPBP1 [nucleoplasm]

Physical Entity
ERCC1:ERCC4:RAD51:p-Y104-RAD52:p-RPA:ATR:ATRIP:DNA DSBs with annealed 3' ssDNA overhangs and displaced flaps:p-MRN:p-S1981,Ac-K3016-ATM:KAT5:BRCA1-C complex:EXO1,DNA2:BLM,WRN:p-S990,Ac-K1249-BRIP1:RAD17:RFC:RAD9:HUS1:RAD1:RHNO1:TOPBP1 [nucleoplasm] (Homo sapiens)
Activity
DNA endonuclease activity (GO:0004520)
Orthologous Events
ERCC1:XPF cleaves flaps generated by SSA (Bos taurus)
ERCC1:XPF cleaves flaps generated by SSA (Canis familiaris)
ERCC1:XPF cleaves flaps generated by SSA (Danio rerio)
ERCC1:XPF cleaves flaps generated by SSA (Dictyostelium discoideum)
ERCC1:XPF cleaves flaps generated by SSA (Gallus gallus)
ERCC1:XPF cleaves flaps generated by SSA (Mus musculus)
ERCC1:XPF cleaves flaps generated by SSA (Rattus norvegicus)
ERCC1:XPF cleaves flaps generated by SSA (Sus scrofa)
Authored
Orlic-Milacic, M  (2015-05-12)
Reviewed
Borowiec, JA  (2015-06-12)
Created
Orlic-Milacic, M  (2015-04-02)
Cite Us!