ERCC1:XPF cleaves flaps generated by SSA

Stable Identifier
Reaction [transition]
Homo sapiens
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The endonuclease complex ERCC1:XPF (ERCC1:ERCC4) is recruited to single strand annealing (SSA) sites of DNA double-strand break (DSB) repair through direct interaction between XPF (ERCC4) and RAD52 (Motycka et al. 2004). ERCC1:XPF cleaves the ssDNA flaps generated by displacement of non-complementary 3' parts of 3' ssDNA overhangs during RAD52-mediated annealing. The enzymatic activity of ERCC1:XPF is necessary for the completion of SSA (Motycka et al. 2004, Al-Minawi et al. 2008, Ahmad et al. 2008).

Literature References
PubMed ID Title Journal Year
18541667 ERCC1-XPF endonuclease facilitates DNA double-strand break repair

van Drunen, E, Ahmad, A, Beverloo, HB, Niedernhofer, LJ, Robinson, AR, Weisberg, DB, Hasty, P, Duensing, A, Hoeijmakers, JH

Mol. Cell. Biol. 2008
17962301 The ERCC1/XPF endonuclease is required for efficient single-strand annealing and gene conversion in mammalian cells

Saleh-Gohari, N, Helleday, T, Al-Minawi, AZ

Nucleic Acids Res. 2008
14734547 Physical and functional interaction between the XPF/ERCC1 endonuclease and hRad52

Bessho, T, Post, SM, Motycka, TA, Sung, P, Tomkinson, AE

J. Biol. Chem. 2004
Catalyst Activity

endodeoxyribonuclease activity of ERCC1:ERCC4 [nucleoplasm]

Orthologous Events
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