MUS81:EME1,EME2 cleaves D-loop

Stable Identifier
R-HSA-5686440
Type
Reaction [transition]
Species
Homo sapiens
Compartment
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The complex of MUS81 and EME1 or EME2 acts as an endonuclease and processes D-loops by cleaving the sister chromatid strand complementary to the invading strand at the junction point. MUS81:EME2 complex has a higher catalytic activity than MUS81:EME1 complex and, in vitro, cleaves D-loops at several other sites in addition to the junction point (Osman et al. 2003, Pepe and West 2014). The yeast Mus81:Eme1 (Mus81:Mms4) complex also acts on D-loops (Schwartz et al. 2012).

Literature References
PubMed ID Title Journal Year
24371268 Substrate specificity of the MUS81-EME2 structure selective endonuclease

Pepe, A, West, SC

Nucleic Acids Res. 2014
14527420 Generating crossovers by resolution of nicked Holliday junctions: a role for Mus81-Eme1 in meiosis

Osman, F, Dixon, J, Doe, CL, Whitby, MC

Mol. Cell 2003
22645308 Mus81-Mms4 functions as a single heterodimer to cleave nicked intermediates in recombinational DNA repair

Schwartz, EK, Wright, WD, Ehmsen, KT, Evans, JE, Stahlberg, H, Heyer, WD

Mol. Cell. Biol. 2012
Participants
Participant Of
Catalyst Activity
Catalyst Activity
Title
endodeoxyribonuclease activity of MUS81:EME1, (MUS81:EME2) [nucleoplasm]
Physical Entity
Activity
Orthologous Events
Authored
Reviewed
Created