BLM mediates dissolution of double Holliday junction

Stable Identifier
Reaction [transition]
Homo sapiens
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The BTRR complex, composed of BLM, TOP3A, RMI1 and RMI2, dissolves double Holliday junctions by disentangling hemicatenane intermediates (Bocquet et al. 2014). This results in non-crossover products, where no exchange of genetic material happens between the sister chromatid that served as a template for the DNA repair synthesis and the repaired DNA duplex. SPIDR serves as a scaffold that connects the BTRR complex with the double Holliday junction through its simultaneous interaction with RAD51-coated DNA strands of the Holliday junction and BLM. SPIDR is needed for BTRR-mediated prevention of cross-over between sister chromatids (Wan et al. 2013).

Literature References
PubMed ID Title Journal Year
23509288 Scaffolding protein SPIDR/KIAA0146 connects the Bloom syndrome helicase with homologous recombination repair

Chen, H, Huang, J, Liu, T, Han, J, Dong, S, Wan, L, Xie, F

Proc. Natl. Acad. Sci. U.S.A. 2013
24509834 Structural and mechanistic insight into Holliday-junction dissolution by topoisomerase III? and RMI1

Larsen, NB, Hickson, ID, Bizard, AH, Bunker, RD, Abdulrahman, W, Thomä, NH, Cejka, P, Faty, M, Kowalczykowski, SC, Bocquet, N, Cavadini, S

Nat. Struct. Mol. Biol. 2014
Catalyst Activity

helicase activity of BLM:TOP3A:RMI1:RMI2:SPIDR [nucleoplasm]

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