ATR phosphorylates RPA2

Stable Identifier
R-HSA-5685156
Type
Reaction [transition]
Species
Homo sapiens
Compartment
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Activated ATR phosphorylates the RPA2 subunit of the RPA complex on serine residue S33. Phosphorylation of RPA2 at S33 likely stimulates additional RPA2 phosphorylation on CDK sites (S23 and S29) and DNA-PKcs (PRKDC) sites (S4, S8 and S21). DNA damage-regulated phosphorylation of RPA2 plays an important role in the progression of homologous recombination-directed repair of DNA double strand breaks (DSBs) (Anantha et al. 2007, Liu et al. 2012, Murphy et al. 2014)

Literature References
PubMed ID Title Journal Year
22977173 Distinct roles for DNA-PK, ATM and ATR in RPA phosphorylation and checkpoint activation in response to replication stress

Liu, S, Opiyo, SO, Manthey, K, Glanzer, JG, Ashley, AK, Amerin, C, Troksa, K, Shrivastav, M, Nickoloff, JA, Oakley, GG

Nucleic Acids Res. 2012
17928296 Sequential and synergistic modification of human RPA stimulates chromosomal DNA repair

Anantha, RW, Vassin, VM, Borowiec, JA

J Biol Chem 2007
25113031 Phosphorylated RPA recruits PALB2 to stalled DNA replication forks to facilitate fork recovery

Murphy, AK, Fitzgerald, M, Ro, T, Kim, JH, Rabinowitsch, AI, Chowdhury, D, Schildkraut, CL, Borowiec, JA

J. Cell Biol. 2014
Participants
Participant Of
Catalyst Activity
Catalyst Activity
Title
protein serine/threonine kinase activity of ATR:ATRIP:RPA:3' overhanging ssDNA-DSBs:p-MRN:p-S1981,Ac-K3016-ATM:KAT5:BRCA1-C complex:EXO1,DNA2:BLM,WRN:p-S990,Ac-K1249-BRIP1:RAD17:RFC:RAD9:HUS1:RAD1:RHNO1:TOPBP1 [nucleoplasm]
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Activity
Orthologous Events
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Created