ATR phosphorylates RPA2

Stable Identifier
Reaction [transition]
Homo sapiens
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Activated ATR phosphorylates the RPA2 subunit of the RPA complex on serine residue S33. Phosphorylation of RPA2 at S33 likely stimulates additional RPA2 phosphorylation on CDK sites (S23 and S29) and DNA-PKcs (PRKDC) sites (S4, S8 and S21). DNA damage-regulated phosphorylation of RPA2 plays an important role in the progression of homologous recombination-directed repair of DNA double-strand breaks (DSBs) (Anantha et al. 2007, Liu et al. 2012, Murphy et al. 2014)

Literature References
PubMed ID Title Journal Year
17928296 Sequential and synergistic modification of human RPA stimulates chromosomal DNA repair

Borowiec, JA, Vassin, VM, Anantha, RW

J Biol Chem 2007
22977173 Distinct roles for DNA-PK, ATM and ATR in RPA phosphorylation and checkpoint activation in response to replication stress

Troksa, K, Shrivastav, M, Amerin, C, Nickoloff, JA, Oakley, GG, Liu, S, Ashley, AK, Glanzer, JG, Manthey, K, Opiyo, SO

Nucleic Acids Res. 2012
25113031 Phosphorylated RPA recruits PALB2 to stalled DNA replication forks to facilitate fork recovery

Borowiec, JA, Rabinowitsch, AI, Murphy, AK, Schildkraut, CL, Fitzgerald, M, Chowdhury, D, Kim, JH, Ro, T

J. Cell Biol. 2014
Catalyst Activity

protein serine/threonine kinase activity of ATR:ATRIP:RPA:3' overhanging ssDNA-DSBs:p-MRN:p-S1981,Ac-K3016-ATM:KAT5:BRCA1-C complex:EXO1,DNA2:BLM,WRN:p-S990,Ac-K1249-BRIP1:RAD17:RFC:RAD9:HUS1:RAD1:RHNO1:TOPBP1 [nucleoplasm]

Orthologous Events
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