IKBKB phosphorylates TPL2 (MAP3K8) at Ser400

Stable Identifier
R-HSA-5684275
Type
Reaction [transition]
Species
Homo sapiens
Compartment
cytosol
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IKBKB phosphorylates TPL2 (MAP3K8) at Ser400
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The activity of tumor progression locus-2 (TPL2, also known as COT and MAP3K8) is regulated by means of phosphorylation. MAP3K8 undergoes phosphorylated on S400 in its C-terminal tail to activate MAP2Ks (MEK1/2) following LPS stimulation of macrophages. Different experimental systems have suggested that S400 is either autophosphosphorylated by MAPK3P8 (IL-1?-stimulated IL-1R-293T cells) or transphosphorylated by an unknown kinase (LPS-stimulated RAW264.7 macrophages).

Literature References
PubMed ID Title Journal Year
22988300 I?B kinase 2 regulates TPL-2 activation of extracellular signal-regulated kinases 1 and 2 by direct phosphorylation of TPL-2 serine 400

Roget, K, Ben-Addi, A, Mambole-Dema, A, Gantke, T, Yang, HT, Janzen, J, Morrice, N, Abbott, DW, Ley, SC

Mol. Cell. Biol. 2012
19754427 IRAK1-independent pathways required for the interleukin-1-stimulated activation of the Tpl2 catalytic subunit and its dissociation from ABIN2

Handoyo, H, Stafford, MJ, McManus, E, Baltzis, D, Peggie, M, Cohen, P

Biochem. J. 2009
16806191 Interleukin-1 stimulated activation of the COT catalytic subunit through the phosphorylation of Thr290 and Ser62

Stafford, MJ, Morrice, NA, Peggie, MW, Cohen, P

FEBS Lett. 2006
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Catalyst Activity
Catalyst Activity
Title
IkappaB kinase activity of IKBKG:p-S176,S180-CHUK:p-S177,S181-IKBKB [cytosol]
Physical Entity
IKBKG:p-S176,S180-CHUK:p-S177,S181-IKBKB [cytosol]
Activity
IkappaB kinase activity (0008384)
Orthologous Events
IKBKB phosphorylates TPL2 (MAP3K8) at Ser400 (Bos taurus)
IKBKB phosphorylates TPL2 (MAP3K8) at Ser400 (Danio rerio)
IKBKB phosphorylates TPL2 (MAP3K8) at Ser400 (Mus musculus)
IKBKB phosphorylates TPL2 (MAP3K8) at Ser400 (Rattus norvegicus)
IKBKB phosphorylates TPL2 (MAP3K8) at Ser400 (Sus scrofa)
Authored
Shamovsky, V  (2015-05-13)
Reviewed
Jupe, S  (2015-04-14)
DeCicco-Skinner, KL  (2015-08-21)
Created
Shamovsky, V  (2015-03-20)