ATG12 is transferred from ATG10, becoming conjugated to the ATG5 at Lys-130 through an isopeptide bond (Mizushima et al. 1998) that requires no E3-like enzyme. Nuclear magnetic resonance experiments, mutational analyses and crosslinking experiments show that ATG10 directly recognizes ATG5, binding it to place the side chain of ATG5 Lys-130 into an optimal orientation for its conjugation reaction with ATG12, thereby enabling ATG10 to mediate the formation of the ATG12-ATG5 conjugate without a specific E3 enzyme (Yamaguchi et al. 2012).
Shima, T, Inagaki, F, Ohsumi, Y, Akada, R, Yamamoto, H, Kumeta, H, Noda, NN, Yamaguchi, M, Kobashigawa, Y
Gao, Z, Shao, Y, Feldman, T, Jiang, X
© 2023 Reactome
