Reactome | ATG10 dissociates from ATG12:ATG5

ATG10 dissociates from ATG12:ATG5

Stable Identifier

R-HSA-5682690

Type

Reaction [dissociation]

Species

Homo sapiens

Compartment

cytosol

ReviewStatus

5/5

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ATG12 is transferred from ATG10, becoming conjugated to the target protein ATG5 at Lys-149 through an isopeptide bond (Mizushima et al. 1998). There is no E3-like enzyme involved in Atg12-Atg5 conjugation. Nuclear magnetic resonance experiments, mutational analyses, and crosslinking experiments showed that ATG10 directly recognizes ATG5, binding it to place the side chain of ATG5 Lys-145 into an optimal orientation for its conjugation reaction with ATG12, thereby enabling ATG10 to mediate the formation of the ATG12-ATG5 conjugate without a specific E3 enzyme (Yamaguchi et al. 2012).

Literature References

PubMed ID	Title	Journal	Year
16963840	Stimulation of ATG12-ATG5 conjugation by ribonucleic acid Gao, Z, Shao, Y, Feldman, T, Jiang, X	Autophagy	2007
22682742	Structural insights into Atg10-mediated formation of the autophagy-essential Atg12-Atg5 conjugate Shima, T, Inagaki, F, Ohsumi, Y, Akada, R, Yamamoto, H, Kumeta, H, Noda, NN, Yamaguchi, M, Kobashigawa, Y	Structure	2012