ATG10 dissociates from ATG12:ATG5

Stable Identifier
Reaction [dissociation]
Homo sapiens
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ATG12 is transferred from ATG10, becoming conjugated to the target protein ATG5 at Lys-149 through an isopeptide bond (Mizushima et al. 1998). There is no E3-like enzyme involved in Atg12-Atg5 conjugation. Nuclear magnetic resonance experiments, mutational analyses, and crosslinking experiments showed that ATG10 directly recognizes ATG5, binding it to place the side chain of ATG5 Lys-145 into an optimal orientation for its conjugation reaction with ATG12, thereby enabling ATG10 to mediate the formation of the ATG12-ATG5 conjugate without a specific E3 enzyme (Yamaguchi et al. 2012).

Literature References
PubMed ID Title Journal Year
22682742 Structural insights into Atg10-mediated formation of the autophagy-essential Atg12-Atg5 conjugate

Yamaguchi, M, Noda, NN, Yamamoto, H, Shima, T, Kumeta, H, Kobashigawa, Y, Akada, R, Ohsumi, Y, Inagaki, F

Structure 2012
16963840 Stimulation of ATG12-ATG5 conjugation by ribonucleic acid

Shao, Y, Gao, Z, Feldman, T, Jiang, X

Autophagy 2007
Participant Of
Orthologous Events
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