Reactome | PIAS4 SUMOylates HERC2 with SUMO1 at DNA DSBs

PIAS4 SUMOylates HERC2 with SUMO1 at DNA DSBs

Stable Identifier

R-HSA-5682607

Type

Reaction [transition]

Species

Homo sapiens

Compartment

nucleoplasm

ReviewStatus

5/5

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PIAS4 SUMOylates HERC2 with SUMO1 at DNA DSBs

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PIAS4 SUMOylates HERC2 at an unknown lysine residue with SUMO1. ATM activity is needed for PIAS4-mediated HERC2 SUMOylation and it is therefore plausible that the ATM-mediated phosphorylation of HERC2 precedes HERC2 SUMOylation. Once SUMOylated, the ZZ domain of HERC2 interacts with the attached SUMO1 group, probably leading to a conformational change that allows binding of phosphorylated HERC2 to RNF8 (Bekker-Jensen et al. 2010, Danielsen et al. 2012).

Literature References

PubMed ID	Title	Journal	Year
20023648	HERC2 coordinates ubiquitin-dependent assembly of DNA repair factors on damaged chromosomes Lukas, J, Gromova, I, Rendtlew Danielsen, J, Bartek, J, Nerstedt, A, Lukas, C, Fugger, K, Bekker-Jensen, S, Mailand, N	Nat. Cell Biol.	2010
22508508	DNA damage-inducible SUMOylation of HERC2 promotes RNF8 binding via a novel SUMO-binding Zinc finger Wikström, M, Nilsson, J, Povlsen, LK, Danielsen, JR, Streicher, W, Bekker-Jensen, S, Villumsen, BH, Mailand, N	J. Cell Biol.	2012

Participants

Input

Output

Participates

as an event of

Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks (Homo sapiens)

Catalyst Activity

SUMO transferase activity of DNA DSBs:p-MRN:p-S1981,Ac-K3016-ATM:KAT5:p-S139-H2AFX,Me2K21-HIST1H4A-Nucleosome:p-5T-MDC1:KDM4A,B:p-S102-WHSC1:RNF8:Zn2+:p-T4827-HERC2:PIAS4 [nucleoplasm]

Physical Entity

DNA DSBs:p-MRN:p-S1981,Ac-K3016-ATM:KAT5:p-S139-H2AFX,Me2K21-HIST1H4A-Nucleosome:p-5T-MDC1:KDM4A,B:p-S102-WHSC1:RNF8:Zn2+:p-T4827-HERC2:PIAS4 [nucleoplasm] (Homo sapiens)

Activity

SUMO transferase activity (GO:0019789)

Orthologous Events

PIAS4 SUMOylates HERC2 with SUMO1 at DNA DSBs (Bos taurus)

PIAS4 SUMOylates HERC2 with SUMO1 at DNA DSBs (Gallus gallus)

PIAS4 SUMOylates HERC2 with SUMO1 at DNA DSBs (Mus musculus)

PIAS4 SUMOylates HERC2 with SUMO1 at DNA DSBs (Rattus norvegicus)

PIAS4 SUMOylates HERC2 with SUMO1 at DNA DSBs (Saccharomyces cerevisiae)

PIAS4 SUMOylates HERC2 with SUMO1 at DNA DSBs (Schizosaccharomyces pombe)

PIAS4 SUMOylates HERC2 with SUMO1 at DNA DSBs (Sus scrofa)