Activated RAF phosphorylates the MEK kinases MAP2K1 and MAP2K2 on 2 serine residues in the MAP2K activation loop (S218 and S222 in MAP2K1 and S222 and S226 in MAP2K2 (Zheng and Guan, 1994; Alessi et al, 1994; Catling et al, 1995; Papin et al, 1995; Seger et al, 1994; reviewed in Roskoski, 2012a). Although all three RAF kinases can phosphorylate MAP2K1 and MAP2K2, BRAF appears to be the primary activator in vivo (Marais et al, 1997; Jaiswal et al, 1994; Pritchard et al, 1995; reviewed in Welbrock et al, 2004)
Roskoski, R Jr
Bosch, E, McMahon, M, Samuels, ML, Pritchard, CA
Seger, D, Reszka, AA, Seger, R, Krebs, EG, Campbell, JS, Fischer, EH, Dobrowolska, G, Jensen, AM, Munar, ES, Eldar-Finkelman, H
Reddy, GR, Weber, MJ, Schaeffer, HJ, Reuter, CW, Catling, AD
Barnier, JV, Pouysségur, J, Calothy, G, Brunet, A, Pagès, G, Papin, C, Eychène, A
Paterson, HF, Light, Y, Mason, CS, Marshall, CJ, Marais, R
Moodie, SA, Wolfman, A, Landreth, GE, Jaiswal, RK
Zheng, CF, Guan, KL
Ashworth, A, Campbell, DG, Cowley, S, Cohen, P, Alessi, DR, Marshall, CJ, Sithanandam, G, Rapp, U, Saito, Y
protein serine/threonine kinase activity of activated RAF:scaffold:MAP2K:MAPK complex [plasma membrane]
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