Activated RAF phosphorylates the MEK kinases MAP2K1 and MAP2K2 on 2 serine residues in the MAP2K activation loop (S218 and S222 in MAP2K1 and S222 and S226 in MAP2K2 (Zheng and Guan, 1994; Alessi et al, 1994; Catling et al, 1995; Papin et al, 1995; Seger et al, 1994; reviewed in Roskoski, 2012a). Although all three RAF kinases can phosphorylate MAP2K1 and MAP2K2, BRAF appears to be the primary activator in vivo (Marais et al, 1997; Jaiswal et al, 1994; Pritchard et al, 1995; reviewed in Welbrock et al, 2004)
Pritchard, CA, Samuels, ML, Bosch, E, McMahon, M
Roskoski, R Jr
Seger, R, Seger, D, Reszka, AA, Munar, ES, Eldar-Finkelman, H, Dobrowolska, G, Jensen, AM, Campbell, JS, Fischer, EH, Krebs, EG
Catling, AD, Schaeffer, HJ, Reuter, CW, Reddy, GR, Weber, MJ
Papin, C, Eychène, A, Brunet, A, Pagès, G, Pouysségur, J, Calothy, G, Barnier, JV
Marais, R, Light, Y, Paterson, HF, Mason, CS, Marshall, CJ
Jaiswal, RK, Moodie, SA, Wolfman, A, Landreth, GE
Zheng, CF, Guan, KL
Alessi, DR, Saito, Y, Campbell, DG, Cohen, P, Sithanandam, G, Rapp, U, Ashworth, A, Marshall, CJ, Cowley, S
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