Reactome | MT3 binds zinc

MT3 binds zinc

Stable Identifier

R-HSA-5662606

Type

Reaction [binding]

Species

Homo sapiens

Compartment

cytosol

ReviewStatus

5/5

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The metallothionein MT3 binds 7 zinc(II) atoms in 2 clusters, one cluster of 4 atoms at the C-terminal alpha domain and one cluster of 3 atoms at the N-terminal beta domain (Eriste et al. 2003, Knipp et al. 2005, Meloni et al. 2007, Meloni et al. 2008, Meloni et al. 2009, Wu et al. 2014). Though MT3 has a lower overall affinity for zinc than MT2A does (Palumaa et al. 2005), MT3 is capable of binding an additional zinc atom with a binding constant of 100 micromolar (Eriste et al. 2003, Palumaa et al. 2005, Meloni et al. 2009). Stoichiometries up to MT3:11Zn2+ have been observed but the MT3:7Zn2+ form is more stable and predominates (Palumaa et al. 2002, Eriste et al. 2003, Palumaa et al.).

Literature References

PubMed ID	Title	Journal	Year
15698955	Metal binding of metallothionein-3 versus metallothionein-2: lower affinity and higher plasticity Sillard, R, Jörnvall, H, Kruusel, K, Palumaa, P, Kangur, L, Tammiste, I	Biochim. Biophys. Acta	2005
24973778	High level expression, efficient purification, and bioactivity of recombinant human metallothionein 3 (rhMT3) from methylotrophic yeast Pichia pastoris Li, S, Cui, T, Wu, D, Li, H, Wu, F, Tang, D, Yang, L, Li, B, Wu, Q	Protein Expr. Purif.	2014
15736926	Zn7metallothionein-3 and the synaptic vesicle cycle: interaction of metallothionein-3 with the small GTPase Rab3A Vasák, M, Knipp, M, Roschitzki, B, Meloni, G	Biochemistry	2005
19425569	Effects of Zn(2+), Ca(2+), and Mg(2+) on the structure of Zn(7)metallothionein-3: evidence for an additional zinc binding site Vasák, M, Braun, O, Meloni, G, Polanski, T	Biochemistry	2009
17389590	Redox silencing of copper in metal-linked neurodegenerative disorders: reaction of Zn7metallothionein-3 with Cu2+ ions Vasák, M, Faller, P, Meloni, G	J. Biol. Chem.	2007
18454142	Metal swap between Zn7-metallothionein-3 and amyloid-beta-Cu protects against amyloid-beta toxicity Guilloreau, L, Gillet, A, Vasák, M, Teissié, J, Faller, P, Delaine, T, Meloni, G, Sonois, V	Nat. Chem. Biol.	2008
11994011	Brain-specific metallothionein-3 has higher metal-binding capacity than ubiquitous metallothioneins and binds metals noncooperatively Sillard, R, Njunkova, O, Jörnvall, H, Palumaa, P, Pokras, L, Eriste, E	Biochemistry	2002
12963354	Purification of recombinant human apometallothionein-3 and reconstitution with zinc Sillard, R, Jörnvall, H, Palumaa, P, Kruusel, K, Eriste, E	Protein Expr. Purif.	2003