ALKBH3 associated with ASCC1:ASCC2:ASCC3 binds alkylated dsDNA containing 3-meC

Stable Identifier
R-HSA-5657617
Type
Reaction [binding]
Species
Homo sapiens
Compartment
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ALKBH3 (ABH3) has a preference for binding single strand DNA or RNA containing alkylation damage. ALKBH3 associates with dsDNA containing 3-methylcytosine (3-meC-dsDNA) alkylation damage in the presence of ASCC3 DNA helicase. ASCC3 is a part of ASCC1:ASCC2:ASCC3 activating signal co-integrator complex, which unwinds dsDNA, providing an appropriate substrate for ALKBH3 (Duncan et al. 2002, Sundheim et al. 2006, Chen et al. 2010, Dango et al. 2011). ALKBH3 requires iron (Fe2+) for its catalytic activity (Duncan et al. 2002, Sundheim et al. 2006). ALKBH3 is ~2-fold more active on DNA containing 3-methylcytosine than 1-methyladenine (Duncan et al. 2002).

Literature References
PubMed ID Title Journal Year
22055184 DNA unwinding by ASCC3 helicase is coupled to ALKBH3-dependent DNA alkylation repair and cancer cell proliferation

Dango, S, Mosammaparast, N, Sowa, ME, Xiong, LJ, Wu, F, Park, K, Rubin, M, Gygi, S, Harper, JW, Shi, Y

Mol. Cell 2011
20714506 Mechanistic insight into the recognition of single-stranded and double-stranded DNA substrates by ABH2 and ABH3

Chen, B, Liu, H, Sun, X, Yang, CG

Mol Biosyst 2010
16858410 Human ABH3 structure and key residues for oxidative demethylation to reverse DNA/RNA damage

Sundheim, O, Vågbø, CB, Bjørås, M, Sousa, MM, Talstad, V, Aas, PA, Drabløs, F, Krokan, HE, Tainer, JA, Slupphaug, G

EMBO J. 2006
12486230 Reversal of DNA alkylation damage by two human dioxygenases.

Duncan, T, Trewick, SC, Koivisto, P, Bates, PA, Lindahl, T, Sedgwick, B

Proc Natl Acad Sci U S A 2002
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