Reactome | RCHY1 monoubiquitinates POLH

RCHY1 monoubiquitinates POLH

Stable Identifier

R-HSA-5655170

Type

Reaction [transition]

Species

Homo sapiens

Compartment

nucleoplasm

ReviewStatus

5/5

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RCHY1 (Pirh2) acts as an E3 ubiquitin ligase to monoubiquitinate POLH (DNA polymerase eta) on lysine residues K682, K686, K694 and K709 located in the NLS (nuclear localization signal) of POLH (Jung et al. 2011). The NLS sequence of POLH is located between UBZ domain and PIP box, involved in POLH binding to monoubiquitinated PCNA (MonoUb:K164-PCNA). POLH monoubiquitination masks the PCNA-interaction region, thus disabling POLH binding to MonoUb:K164-PCNA and preventing POLH-mediated translesion DNA synthesis (TLS) (Bienko et al. 2010).

Literature References

PubMed ID	Title	Journal	Year
21791603	Pirh2 E3 ubiquitin ligase monoubiquitinates DNA polymerase eta to suppress translesion DNA synthesis Jung, YS, Hakem, A, Hakem, R, Chen, X	Mol. Cell. Biol.	2011
20159558	Regulation of translesion synthesis DNA polymerase eta by monoubiquitination Bienko, M, Green, CM, Sabbioneda, S, Crosetto, N, Matic, I, Hibbert, RG, Begovic, T, Niimi, A, Mann, M, Lehmann, AR, Dikic, I	Mol. Cell	2010