RCHY1 monoubiquitinates POLH

Stable Identifier
Reaction [transition]
Homo sapiens
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RCHY1 (Pirh2) acts as an E3 ubiquitin ligase to monoubiquitinate POLH (DNA polymerase eta) on lysine residues K682, K686, K694 and K709 located in the NLS (nuclear localization signal) of POLH (Jung et al. 2011). The NLS sequence of POLH is located between UBZ domain and PIP box, involved in POLH binding to monoubiquitinated PCNA (MonoUb:K164-PCNA). POLH monoubiquitination masks the PCNA-interaction region, thus disabling POLH binding to MonoUb:K164-PCNA and preventing POLH-mediated translesion DNA synthesis (TLS) (Bienko et al. 2010).

Literature References
PubMed ID Title Journal Year
21791603 Pirh2 E3 ubiquitin ligase monoubiquitinates DNA polymerase eta to suppress translesion DNA synthesis

Hakem, R, Chen, X, Hakem, A, Jung, YS

Mol. Cell. Biol. 2011
20159558 Regulation of translesion synthesis DNA polymerase eta by monoubiquitination

Begovic, T, Crosetto, N, Green, CM, Lehmann, AR, Niimi, A, Sabbioneda, S, Matic, I, Dikic, I, Mann, M, Hibbert, RG, Bienko, M

Mol. Cell 2010
Catalyst Activity

ubiquitin-protein transferase activity of POLH:RCHY1 [nucleoplasm]

Orthologous Events
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