UBE2L6:TRIM25 ISGylates monoUb:K164-PCNA

Stable Identifier
R-HSA-5653754
Type
Reaction [transition]
Species
Homo sapiens
Compartment
nucleoplasm
ReviewStatus
5/5
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UBE2L6:TRIM25 ISGylates monoUb:K164-PCNA
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The complex of ISG15 E2 conjugating enzyme UBE2L6 (UBCH8) and ISG15 E3 conjugating enzyme TRIM25 (EFP) doubly ISGylates monoubiquitinated PCNA (MonoUb:K164-PCNA) on lysine residues K164 and K168. Note that PCNA is a homotrimer, meaning ISGylation of K164 (and K168) occurs on a different subunit than that monoubiquitinated on K164. UBA7 (UBE1L) is the ISG15 E1-activating enzyme (Park et al. 2014).
Literature References
PubMed ID Title Journal Year
24768535 Modification of PCNA by ISG15 plays a crucial role in termination of error-prone translesion DNA synthesis

Lee, SW, Seol, JH, Yang, SW, Yu, KR, Park, JM, Ka, SH, Jeon, YJ, Chung, CH

Mol. Cell 2014
Participants
Input
Output
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as an event of
Catalyst Activity

ISG15 transferase activity of UBE2L6:TRIM25:DNA polymerase Y:MonoUb:K164-PCNA:RPA:RFC:TLS-DNA Template [nucleoplasm]

Physical Entity
UBE2L6:TRIM25:DNA polymerase Y:MonoUb:K164-PCNA:RPA:RFC:TLS-DNA Template [nucleoplasm] (Homo sapiens)
Activity
ISG15 transferase activity (GO:0042296)
Orthologous Events
UBE2L6:TRIM25 ISGylates monoUb:K164-PCNA (Bos taurus)
UBE2L6:TRIM25 ISGylates monoUb:K164-PCNA (Mus musculus)
UBE2L6:TRIM25 ISGylates monoUb:K164-PCNA (Rattus norvegicus)
UBE2L6:TRIM25 ISGylates monoUb:K164-PCNA (Sus scrofa)
Authored
Orlic-Milacic, M  (2014-12-11)
Reviewed
Borowiec, JA  (2015-01-07)
Created
Orlic-Milacic, M  (2014-12-02)
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