LIG1 bound to APEX1 and PCNA ligates SSB

Stable Identifier
R-HSA-5651805
Type
Reaction [transition]
Species
Homo sapiens
Compartment
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LIG1 (DNA ligase 1) is recruited to the long-patch base excision repair site through its interaction with APEX1, PCNA and RFC. LIG1 ligates the 3' end of the single strand break (SSB), containing the newly synthesized multiple nucleotide repair patch, with the FEN1-processed 5' end of the SSB, thus completing the base excision repair (Klungland and Lindahl 1997, Tomkinson et al. 2001, Ranalli et al. 2002a). The catalytic activity of LIG1 is stimulated by the presence of PCNA-bound RPA (Ranalli et al. 2002b)

Literature References
PubMed ID Title Journal Year
11698410 Mechanism underlying replication protein a stimulation of DNA ligase I

Ranalli, TA, DeMott, MS, Bambara, RA

J. Biol. Chem. 2002
16464020 DNA ligases: structure, reaction mechanism, and function

Tomkinson, AE, Vijayakumar, S, Pascal, JM, Ellenberger, T

Chem. Rev. 2006
12200445 AP endonuclease 1 coordinates flap endonuclease 1 and DNA ligase I activity in long patch base excision repair.

Ranalli, TA, Tom, S, Bambara, RA

J Biol Chem 2002
9214649 Second pathway for completion of human DNA base excision-repair: reconstitution with purified proteins and requirement for DNase IV (FEN1).

Klungland, A, Lindahl, T

EMBO J 1997
Participants
Participant Of
Catalyst Activity
Catalyst Activity
Title
DNA ligase activity of LIG1:APEX1:PCNA:POLD,POLE:RPA:RFC:SSB:(3'poly-dNMP)-dsDNA [nucleoplasm]
Physical Entity
Activity
Orthologous Events
Authored
Reviewed
Created