Reactome | POLB excises the NEIL1,NEIL2-bound AP site (5'dRP)

POLB excises the NEIL1,NEIL2-bound AP site (5'dRP)

Stable Identifier

R-HSA-5649725

Type

Reaction [transition]

Species

Homo sapiens

Compartment

nucleoplasm

ReviewStatus

5/5

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POLB excises the NEIL1,NEIL2-bound AP site (5'dRP)

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POLB (DNA polymerase beta) acts as a 5'-exonuclease to remove the abasic deoxyribose residue (5'dRP) generated by the DNA glycosylase and/or beta/delta lyase activity of NEIL1 or NEIL2, producing a single nucleotide gap at the SSB (single strand break) site (Wiederhold et al. 2004, Das et al. 2006). The NEIL1 or NEIL2-generated SSB has a 3' terminal phosphate (3'Pi).

Literature References

PubMed ID	Title	Journal	Year
16982218	NEIL2-initiated, APE-independent repair of oxidized bases in DNA: Evidence for a repair complex in human cells Das, A, Wiederhold, L, Leppard, JB, Kedar, P, Prasad, R, Wang, H, Boldogh, I, Karimi-Busheri, F, Weinfeld, M, Tomkinson, AE, Wilson, SH, Mitra, S, Hazra, TK	DNA Repair (Amst.)	2006
15260972	AP endonuclease-independent DNA base excision repair in human cells Wiederhold, L, Leppard, JB, Kedar, P, Karimi-Busheri, F, Rasouli-Nia, A, Weinfeld, M, Tomkinson, AE, Izumi, T, Prasad, R, Wilson, SH, Mitra, S, Hazra, TK	Mol. Cell	2004