POLB (DNA polymerase beta) acts as a 5'-exonuclease to remove the abasic deoxyribose residue (5'dRP) generated by the DNA glycosylase and/or beta/delta lyase activity of NEIL1 or NEIL2, producing a single nucleotide gap at the SSB (single strand break) site (Wiederhold et al. 2004, Das et al. 2006). The NEIL1 or NEIL2-generated SSB has a 3' terminal phosphate (3'Pi).
Karimi-Busheri, F, Wang, H, Weinfeld, M, Mitra, S, Hazra, TK, Kedar, P, Wilson, SH, Leppard, JB, Prasad, R, Boldogh, I, Tomkinson, AE, Wiederhold, L, Das, A
Karimi-Busheri, F, Weinfeld, M, Mitra, S, Wiederhold, L, Izumi, T, Hazra, TK, Kedar, P, Wilson, SH, Leppard, JB, Prasad, R, Rasouli-Nia, A, Tomkinson, AE
DNA-(apurinic or apyrimidinic site) endonuclease activity of NEIL1:NEIL2:POLB:SSB(3'Pi-5'dRP)-dsDNA [nucleoplasm]
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