POLB incorporates a single nucleotide in place of excised AP residue in NEIL1,NEIL2-mediated AP site resolution

Stable Identifier
R-HSA-5649723
Type
Reaction [transition]
Species
Homo sapiens
Compartment
nucleoplasm
ReviewStatus
5/5
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POLB incorporates a single nucleotide in place of excised AP residue in NEIL1,NEIL2-mediated AP site resolution
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POLB (DNA polymerase beta) incorporates a single nucleotide to extend the PNKP-processed 3' end of the single strand break (SSB) generated by the beta/delta lyase activity of NEIL1 or NEIL2. POLB thus replaces the excised AP (apurinic/apyrimidinic) deoxyribose phosphate residue (5'dRP) (Wiederhold et al. 2004, Das et al. 2006).
Literature References
PubMed ID Title Journal Year
16982218 NEIL2-initiated, APE-independent repair of oxidized bases in DNA: Evidence for a repair complex in human cells

Das, A, Wiederhold, L, Leppard, JB, Kedar, P, Prasad, R, Wang, H, Boldogh, I, Karimi-Busheri, F, Weinfeld, M, Tomkinson, AE, Wilson, SH, Mitra, S, Hazra, TK

DNA Repair (Amst.) 2006
15260972 AP endonuclease-independent DNA base excision repair in human cells

Wiederhold, L, Leppard, JB, Kedar, P, Karimi-Busheri, F, Rasouli-Nia, A, Weinfeld, M, Tomkinson, AE, Izumi, T, Prasad, R, Wilson, SH, Mitra, S, Hazra, TK

Mol. Cell 2004
Participants
Input
Output
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as an event of
Catalyst Activity

DNA-directed DNA polymerase activity of NEIL1,NEIL2:POLB:LIG3:XRCC1:PNKP:SSB-gap-dsDNA [nucleoplasm]

Physical Entity
NEIL1,NEIL2:POLB:LIG3:XRCC1:PNKP:SSB-gap-dsDNA [nucleoplasm] (Homo sapiens)
Activity
DNA-directed DNA polymerase activity (GO:0003887)
Orthologous Events
POLB incorporates a single nucleotide in place of excised AP residue in NEIL1,NEIL2-mediated AP site resolution (Bos taurus)
POLB incorporates a single nucleotide in place of excised AP residue in NEIL1,NEIL2-mediated AP site resolution (Canis familiaris)
POLB incorporates a single nucleotide in place of excised AP residue in NEIL1,NEIL2-mediated AP site resolution (Danio rerio)
POLB incorporates a single nucleotide in place of excised AP residue in NEIL1,NEIL2-mediated AP site resolution (Dictyostelium discoideum)
POLB incorporates a single nucleotide in place of excised AP residue in NEIL1,NEIL2-mediated AP site resolution (Mus musculus)
POLB incorporates a single nucleotide in place of excised AP residue in NEIL1,NEIL2-mediated AP site resolution (Rattus norvegicus)
POLB incorporates a single nucleotide in place of excised AP residue in NEIL1,NEIL2-mediated AP site resolution (Sus scrofa)
POLB incorporates a single nucleotide in place of excised AP residue in NEIL1,NEIL2-mediated AP site resolution (Xenopus tropicalis)
Authored
Orlic-Milacic, M  (2014-12-04)
Reviewed
Borowiec, JA  (2014-12-22)
Created
Orlic-Milacic, M  (2014-11-21)
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