Reactome | PNKP hydrolyzes the terminal 3'Pi at the NEIL1,NEIL2-generated single strand break (SSB)

PNKP hydrolyzes the terminal 3'Pi at the NEIL1,NEIL2-generated single strand break (SSB)

Stable Identifier

R-HSA-5649705

Type

Reaction [transition]

Species

Homo sapiens

Compartment

nucleoplasm

ReviewStatus

5/5

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PNKP hydrolyzes the terminal 3'Pi at the NEIL1,NEIL2-generated single strand break (SSB)

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PNKP, a bifunctional polynucleotide phosphatase/kinase, acts as a 3' phosphatase to remove the terminal 3' phosphate group (3'Pi) at the single strand break (SSB) generated by NEIL1 or NEIL2 beta/delta lyase activity. The presence of XRCC1 is necessary for the 3'-phosphatase activity of PNKP. The removal of the 3'Pi (generating a 3'-OH) makes the DNA with the 3'-end suitable for extension by the DNA polymerase beta (POLB) (Whitehouse et al. 2001, Wiederhold et al. 2004, Das et al. 2006).

Literature References

PubMed ID	Title	Journal	Year
16982218	NEIL2-initiated, APE-independent repair of oxidized bases in DNA: Evidence for a repair complex in human cells Karimi-Busheri, F, Wang, H, Weinfeld, M, Mitra, S, Hazra, TK, Kedar, P, Wilson, SH, Leppard, JB, Prasad, R, Boldogh, I, Tomkinson, AE, Wiederhold, L, Das, A	DNA Repair (Amst.)	2006
11163244	XRCC1 stimulates human polynucleotide kinase activity at damaged DNA termini and accelerates DNA single-strand break repair Taylor, RM, Whitehouse, CJ, Weinfeld, M, Lasko, DD, Zhang, H, Thistlethwaite, A, Caldecott, KW, Karimi-Busheri, F	Cell	2001
15260972	AP endonuclease-independent DNA base excision repair in human cells Karimi-Busheri, F, Weinfeld, M, Mitra, S, Wiederhold, L, Izumi, T, Hazra, TK, Kedar, P, Wilson, SH, Leppard, JB, Prasad, R, Rasouli-Nia, A, Tomkinson, AE	Mol. Cell	2004